In vitro adenosine detection with a diamond-based sensor

• Published in: Diamond and related materials Vol. 15; no. 2; pp. 225 - 228
• Main Authors: Xie, Songtao, Shafer, Geoffrey, Wilson, Christopher G., Martin, Heidi B.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01-02-2006; Elsevier
• Subjects: Applied sciences; Biomedical applications; Chemical vapor deposition (including plasma-enhanced cvd, mocvd, etc.); Corrosion; Corrosion mechanisms; Cross-disciplinary physics: materials science; rheology; Diamond film; Exact sciences and technology; Hot filament CVD; Materials science; Metals. Metallurgy; Methods of deposition of films and coatings; film growth and epitaxy; Physics; Sensors; Biomedical applications; Diamond film; Sensors; Hot filament CVD; Biomedical equipment; Scanning electron microscopy; Adenosine; Breathing rate; Measurement sensor; Doping; Diamond; Crystal growth from vapors; Experimental study; Chemical vapor deposition; Hot wire; Modulation; Microelectrode; Boron addition; Organic compounds; Signal to noise ratio
• ISSN: 0925-9635; 1879-0062
• DOI: 10.1016/j.diamond.2005.08.018

Diamond-based electrodes promise major advantages over present electrode technologies used in biomedical research. To demonstrate these advantages, boron-doped diamond microelectrodes were developed for in vitro detection of adenosine, an important neuromodulator in the central nervous system. Diamond was selectively deposited as part of a unique fabrication strategy to obtain non-planar, needle-like diamond microelectrodes. Preliminary flow injection analysis showed quantified adenosine down to 10 nM, a lower level than the estimated basal adenosine level in vivo, and lower than what is achieved on other electrode materials. As an example of in vitro application, the diamond probe was used to investigate the role of adenosine in respiratory rhythmogenesis (i.e., the modulation of one's breathing rate).