Screening antimutagenic and antiproliferative properties of extracts isolated from Jackfruit pulp (Artocarpus heterophyllus Lam)

• Published in: Food chemistry Vol. 175; pp. 409 - 416
• Main Authors: Ruiz-Montañez, G., Burgos-Hernández, A., Calderón-Santoyo, M., López-Saiz, C.M., Velázquez-Contreras, C.A., Navarro-Ocaña, A., Ragazzo-Sánchez, J.A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15-05-2015
• Subjects: Animals; Antimutagenic; Antimutagenic Agents - pharmacology; Antineoplastic Agents - pharmacology; Antioxidant; Antioxidants - pharmacology; Antiproliferative; Artocarpus - chemistry; Cell Line, Tumor; Chemoprotective properties; Chromatography, High Pressure Liquid; Drug Evaluation, Preclinical; Drug Screening Assays, Antitumor; Jackfruit pulp; Male; Mice; Mutagenicity Tests; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; RP-HPLC; Antiproliferative; RP-HPLC; Antimutagenic; Antioxidant; Jackfruit pulp; Chemoprotective properties
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2014.11.122

•Jackfruit pulp extracts reduced the number of revertants caused by AFB1.•Jackfruit pulp extracts reduced the proliferation of cells M12.C3.F6.•RP-HPLC fractionation of active extracts produced antimutagenic fractions.•RP-HPLC fractionation of active extracts produced antiproliferative fractions.•Jackfruit pulp contains compounds that could prevent or treat lymphoma cancer.
The present focused on the study of the antimutagenic and antiproliferative potential of pulp Jackfruit (Artocarpus heterophyllus Lam) extract, using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line M12.C3.F6 (murine B-cell lymphoma), respectively. Jackfruit pulp extract was sequentially fractionated by chromatography (RP-HPLC) and each fraction was tested for antimutagenic and antiproliferative activities. The organic extracts obtained from Jackfruit pulp reduced the number of revertants caused by aflatoxin B1 (AFB1) and proliferation of cells M12.C3.F6; a dose-response relationship was showed. Sequential RP-HPLC fractionation of the active extracts produced both antimutagenic and/or antiproliferative fractions. These results suggested that the Jackfruit contained compounds with chemoprotective properties to reduce the mutagenicity of AFB1, also proliferation of a cancer cell line.