Metabolism by rat hepatic microsomes of individual isomers and congeners in aroclor 1016

• Published in: Bulletin of environmental contamination and toxicology Vol. 46; no. 3; pp. 436 - 441
• Main Authors: BORLAKOGLU, J. T, WILKINS, J. P. G, KILS, R. R
• Format: Journal Article
• Language: English
• Published: New York, NY Springer-Verlag 01-03-1991
• Subjects: 560300 - Chemicals Metabolism & Toxicology; ADIPOSE TISSUE; ANIMAL TISSUES; ANIMALS; Aroclors - metabolism; AROMATICS; Biological and medical sciences; BODY; CELL CONSTITUENTS; Chemical and industrial products toxicology. Toxic occupational diseases; CHLORINATED AROMATIC HYDROCARBONS; CONNECTIVE TISSUE; DIGESTIVE SYSTEM; Female; GLANDS; HALOGENATED AROMATIC HYDROCARBONS; Hydrogen-Ion Concentration; IN VITRO; In Vitro Techniques; IN VIVO; Isomerism; LIVER; MAMMALS; Medical sciences; METABOLISM; MICROSOMES; Microsomes, Liver - metabolism; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANS; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RATS; Rats, Inbred Strains; RIBOSOMES; RODENTS; TISSUES; Toxicology; Various organic compounds; VERTEBRATES; Vertebrata; Mammalia; Rat; Analog; Animal; Liver; Structure metabolism relation; Rodentia; Metabolism; Polychlorobiphenyl; In vitro
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/BF01688944

Twelve PCB congeners were identified in Aroclor 1016 by high-performance capillary gas chromatography. The microsomal fraction of rat hepatic homogenate was incubated with Aroclor 1016 for 2 h. Gas chromatography indicated that the compounds had metabolized to monohydroxylated entities, but the positions of hydroxy groups could not be determined. In general, congeners without hydrogen pairs at the meta- and para-positions did not metabolize, confirming results of an unpublished in vivo study of PCB accumulation by the same group. The usefulness of the chromatographic method in tracking PCB metabolism is demonstrated.