Caenibacillus caldisaponilyticus gen. nov., sp. nov., a thermophilic, spore-forming and phospholipid-degrading bacterium isolated from acidulocompost

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 7; pp. 2684 - 2690
• Main Authors: Tsujimoto, Yoshiyuki, Saito, Ryo, Furuya, Hiroto, Ishihara, Daisuke, Sahara, Takehiko, Kimura, Nobutada, Nishino, Tokuzo, Tsuruoka, Naoki, Shigeri, Yasushi, Watanabe, Kunihiko
• Format: Journal Article
• Language: English
• Published: England 01-07-2016
• Subjects: Bacillales - chemistry; Bacillales - classification; Bacillales - isolation & purification; Bacillales - physiology; Bacterial Typing Techniques; Base Composition; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fatty Acids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Spores, Bacterial - physiology; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - analysis
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.001108

A thermophilic and phospholipid-degrading bacterium, designated strain B157T, was isolated from acidulocompost, a garbage compost processed under acidic conditions at moderately high temperature. The organism was Gram-stain-positive, aerobic, spore-forming and rod-shaped. Growth was observed to occur at 40-65 °C and pH 4.8-8.1 (optimum growth: 50-60 °C, pH 6.2). The strain was catalase- and oxidase-positive. The cell wall contained meso-diaminopimelic acid, alanine, glutamic acid and galactose. The predominant respiratory quinone was menaquinone-7 (MK-7) and the major fatty acids were anteiso-C17 : 0 and iso-C17 : 0. Comparative 16S rRNA gene sequence analysis showed that strain B157T was related most closely to Tuberibacillus calidus 607T (94.8 % identity), and the phylogenetic analysis revealed that it belonged to the family Sporolactobacillaceae. The DNA G+C content was determined as 51.8 mol%. In spite of many similarities with the type strains of members of the family Sporolactobacillaceae, genotypic analyses suggest that strain B157T represents a novel species of a new genus, Caenibacilluscaldisaponilyticus gen. nov., sp. nov. The type strain of Caenibacilluscaldisaponilyticus is B157T (=NBRC 111400T=DSM 101100T).