Enolase and the arsonomethyl analogue of 2-phosphoglycerate

(RS)-3-Arsono-2-(hydroxymethyl)propionic acid was synthesized by the action of alkaline arsenite on 3-bromo-2-(bromomethyl)propionic acid. It is a substrate for yeast enolase (EC 4.2.1.11) with a Km of 6.5 mM (for 2-phospho-D-glycerate Km = 0.08 mM). The catalytic constant of the enzyme with the ars...

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Bibliographic Details
Published in:	Journal of enzyme inhibition Vol. 8; no. 4; p. 255
Main Authors:	Chawla, S, Dixon, H B
Format:	Journal Article
Language:	English
Published:	Switzerland 1995
Subjects:	Arsenicals - chemical synthesis Arsenicals - metabolism Glyceric Acids - chemical synthesis Glyceric Acids - metabolism Kinetics Phosphopyruvate Hydratase - metabolism Saccharomyces cerevisiae - enzymology Spectrophotometry, Ultraviolet
Online Access:	Get more information
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Summary:	(RS)-3-Arsono-2-(hydroxymethyl)propionic acid was synthesized by the action of alkaline arsenite on 3-bromo-2-(bromomethyl)propionic acid. It is a substrate for yeast enolase (EC 4.2.1.11) with a Km of 6.5 mM (for 2-phospho-D-glycerate Km = 0.08 mM). The catalytic constant of the enzyme with the arsonomethyl analogue is 230 times lower than with 2-phosphoglycerate.
ISSN:	8755-5093
DOI:	10.3109/14756369509020132