Immunocytochemical evidence for the presence of oxytocin and neurophysin in the large cells of the bovine corpus luteum

• Published in: Cell and tissue research Vol. 237; no. 2; pp. 349 - 352
• Main Authors: Guldenaar, S.E.F, Wathes, D.C, Pickering, B.T
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-01-1984
• Subjects: animal anatomy; animal morphology; animal reproduction; Animals; Biological and medical sciences; Cattle; Corpus Luteum - metabolism; Female; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Immunologic Techniques; Mammalian female genital system; Neurophysins - metabolism; Oxytocin - metabolism; Pregnancy; Vasopressins - metabolism; Vertebrates: reproduction; Vertebrata; Mammalia; Corpus luteum; Artiodactyla; Beef; Ungulata; Vasopressin
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/bf00217155

The presence of neurophysin, oxytocin and vasopressin in the bovine corpus luteum was examined immunocytochemically. Tissue blocks of corpora lutea from pregnant and non-pregnant animals were fixed with glutaraldehyde/paraformaldehyde fixative and immunostained by the peroxidase-antiperoxidase (PAP) method. The simultaneous presence of immunoreactive oxytocin and immunoreactive oxytocin-neurophysin was demonstrated in large luteal cells of non-pregnant animals, while no staining for vasopressin or vasopressin-neurophysin was observed. None of the peptides were detected in the corpus luteum of pregnant animals. The small luteal cells were not found to be stainable at any time.