Intracellular degradation of newly synthesized casein in perfused rat mammary gland

Degradation of newly synthesized casein in rat mammary gland perfused in situ was measured by a pulse-chase method using [3H]proline. Casein degradation during secretion was observed in the absence of prolactin, but not with prolactin present. Partial inhibition by chloroquine showed that hormone-de...

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Bibliographic Details
Published in:	Experimental physiology Vol. 76; no. 4; p. 533
Main Authors:	Wilde, C J, Kerr, M A, Calvert, D T
Format:	Journal Article
Language:	English
Published:	England 01-07-1991
Subjects:	Adenosine Triphosphate - metabolism Animals Caseins - antagonists & inhibitors Caseins - metabolism Chloroquine - pharmacology Female Intracellular Membranes - metabolism Mammary Glands, Animal - metabolism Perfusion Prolactin - physiology Rats Rats, Inbred Strains
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Summary:	Degradation of newly synthesized casein in rat mammary gland perfused in situ was measured by a pulse-chase method using [3H]proline. Casein degradation during secretion was observed in the absence of prolactin, but not with prolactin present. Partial inhibition by chloroquine showed that hormone-dependent degradation occurred intracellularly by a lysosomal mechanism. The study indicates that this post-translational mechanism is a physiological regulator of net casein secretion.
ISSN:	0958-0670
DOI:	10.1113/expphysiol.1991.sp003519