Identification of Mimotopes of Platelet Autoantigens Associated with Autoimmune Thrombocytopenic Purpura

• Published in: Journal of autoimmunity Vol. 15; no. 1; pp. 33 - 40
• Main Authors: Gevorkian, G/, Manoutcharian, K., Govezensky, T., Cano, J.A., Dominguez, V., Santamaria, H., Larralde, C.
• Format: Journal Article
• Language: English
• Published: London Elsevier Ltd 01-08-2000; Elsevier
• Subjects: Animals; Autoantibodies, autoantigens, autoimmune thrombocytopenic purpura, phage display peptide library; Autoantigens - analysis; Bacteriophage M13 - immunology; Biological and medical sciences; Blood Platelets - immunology; Enzyme-Linked Immunosorbent Assay; Epitopes - analysis; General aspects; Hematologic and hematopoietic diseases; Humans; Immunopathology; Medical sciences; Mice; Molecular Mimicry; Oligopeptides - analysis; Oligopeptides - immunology; Peptide Library; Peptide Mapping; Platelet diseases and coagulopathies; Platelet Glycoprotein GPIIb-IIIa Complex - immunology; Purpura, Thrombocytopenic, Idiopathic - immunology; Rabbits; Sequence Homology, Amino Acid; Autoantibodies, autoantigens, autoimmune thrombocytopenic purpura, phage display peptide library; Antigen; Autoimmunity; Human; Immunopathology; Peptide library; Glycoprotein IIbIIIa; Phage display; Platelet; Autoantigen; Autoimmune disease; Hemopathy; Immune thrombocytopenic purpura
• ISSN: 0896-8411; 1095-9157
• DOI: 10.1006/jaut.2000.0389

GPIIb/IIIa, the human platelet glycoprotein complex, is the autoantigen most commonly recognized by autoantibodies in autoimmune thrombocytopenic purpura (AITP). Two murine monoclonal antibodies (mAbs), namely Y2/51 and 5B12, directed against gpIIIa and gpIIb/IIIa, respectively, and rabbit anti-human platelet polyclonal antibodies have been used to select AITP-related epitopes from a phage display peptide library expressing random dodecapeptides in the pIII coat protein of M13 phage. The selected phage clones were tested by ELISA for binding to rabbit anti-human platelet antibodies as well as to sera from AITP patients. Seven clones reacted strongly with rabbit anti-human platelet antibodies, and four clones reacted with sera from AITP patients. Some homology between peptide inserts sequences of selected clones and human platelet gpIIIa and gpIb were found.