A mesh microelectrode array for non-invasive electrophysiology within neural organoids

• Published in: Biosensors & bioelectronics Vol. 228; p. 115223
• Main Authors: McDonald, Matthew, Sebinger, David, Brauns, Lisa, Gonzalez-Cano, Laura, Menuchin-Lasowski, Yotam, Mierzejewski, Michael, Psathaki, Olympia-Ekaterini, Stumpf, Angelika, Wickham, Jenny, Rauen, Thomas, Schöler, Hans, Jones, Peter D.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15-05-2023
• Subjects: Biosensing Techniques; Brain organoid; Electrophysiology; Electrophysiology - methods; Humans; In vitro test system; Microelectrode array; Microelectrodes; Microphysiological system; Organoids; Surgical Mesh; Electrophysiology; In vitro test system; Microelectrode array; Brain organoid; Microphysiological system
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2023.115223

Organoids are emerging in vitro models of human physiology. Neural models require the evaluation of functional activity of single cells and networks, which is commonly measured by microelectrode arrays. The characteristics of organoids clash with existing in vitro or in vivo microelectrode arrays. With inspiration from implantable mesh electronics and growth of organoids on polymer scaffolds, we fabricated suspended hammock-like mesh microelectrode arrays for neural organoids. We have demonstrated the growth of organoids enveloping these meshes and the culture of organoids on meshes for up to one year. Furthermore, we present proof-of-principle recordings of spontaneous electrical activity across the volume of an organoid. Our concept enables a new class of microelectrode arrays for in vitro models of three-dimensional electrically active tissue.