Plasmodium chabaudi chabaudi: B-1 Cell Expansion Correlates with Semiresistance in BALB/cJ Mice

• Published in: Experimental parasitology Vol. 98; no. 2; pp. 71 - 82
• Main Authors: Yoder, B.J., Goodrum, K.J.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-06-2001; Elsevier
• Subjects: Animals; Autoantibodies - biosynthesis; B-1 cells; B-1a cells; B-Lymphocyte Subsets - immunology; B1−, B-1 cell depleted; BALB gene; Biological and medical sciences; CPCV2, Plasmodium chabaudi chabaudi; Disease Susceptibility; Experimental protozoal diseases and models; Female; Flow Cytometry; Immunoglobulin M - biosynthesis; Infectious diseases; Malaria - immunology; Malaria - prevention & control; Male; Medical sciences; Mice; Mice, Inbred BALB C; murine malaria; Parasitic diseases; Peritoneal Cavity - cytology; Plasmodium chabaudi - immunology; Plasmodium chabaudi chabaudi; Protozoal diseases; Spleen - cytology; PBS; B1−, B-1 cell depleted; P-Ag; CPCV2, Plasmodium chabaudi chabaudi; IP; IVIG; murine malaria; IV; BSA; OD; ND; HBSS; ssDNA; B-1 cells; dH2 O; ELISA; Cell proliferation; Protozoa; Host parasite relation; Apicomplexa; Protozoal disease; Immune response; Malaria; Rodentia; Host; B-Lymphocyte; Parasitosis; Cell subpopulation; Survival; Plasmodium chabaudi; Infection; Vertebrata; Mammalia; Sensitivity resistance; Mouse; Parasitism; Localization; Expansion; Humoral immunity
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1006/expr.2001.4622

Yoder, B. J., and Goodrum, K. J. 2001. Plasmodium chabaudi chabaudi: B-1 cell expansion correlates with semiresistance in BALB/cJ mice. Experimental Parasitology98, 71–82. The largest obstacle impeding the development of an effective malaria vaccine is the incomplete understanding of how the immune response is regulated during infection. B-1a cells, a poorly understood subcategory of B lymphocytes, produce nonpathologic autoantibodies of low affinity which have been shown to have distinct immunoregulatory capabilities. What the exact activity of B-1a cells are during the course of malaria has yet to be determined. By use of flow cytometry, it was observed that B-1a cells significantly expand by day 3 postinfection in the spleen and peritoneum of Plasmodium chabaudi chabaudi semiresistant BALB/cJ mice, but not until day 8 postinfection in the spleen of P. chabaudi chabaudi fully susceptible BALB/cByJ mice. The activation of B-1a cells was also demonstrated by the measurement of natural autoantibody IgM production from the serum and cultured peritoneal B-1a cells. Infected semiresistant BALB/cJ mice generated higher levels of anti-ssDNA IgM antibodies than infected fully susceptible BALB/cByJ mice. The preliminary data presented here suggest a possible roll of B-1 cells in contributing to the successful survival of murine malarial infection.