A Sequence-Ready Physical Map of the Region Containing the Human Natural Killer Gene Complex on Chromosome 12p12.3–p13.2

• Published in: Genomics (San Diego, Calif.) Vol. 65; no. 2; pp. 129 - 136
• Main Authors: Renedo, Mónica, Arce, Ignacio, Montgomery, Kate, Roda-Navarro, Pedro, Lee, Eunice, Kucherlapati, Raju, Fernández-Ruiz, Elena
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15-04-2000; Elsevier
• Subjects: Antigens, CD - genetics; Antigens, Differentiation, T-Lymphocyte - genetics; Biological and medical sciences; Chromatin. Chromosome; Chromosome Mapping; Chromosomes, Human, Pair 12; DNA Primers; Electrophoresis, Gel, Pulsed-Field; Fundamental and applied biological sciences. Psychology; Gene Library; Humans; Killer Cells, Natural - metabolism; KLR gene; lectins; Lectins - genetics; Lectins, C-Type; Membrane Glycoproteins - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; natural killer cells; NK Cell Lectin-Like Receptor Subfamily D; Polymerase Chain Reaction; Receptors, Cell Surface - genetics; Sequence Tagged Sites; Human; Gene cluster; Targeting; Nucleotide sequence; Glycoprotein; Gene organization; Chromosome C12; Natural killer cell; Contig; Gene; DNA; Molecular complex; Lymphocyte; Sequence tagged site; Physical map; Biological receptor
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.2000.6163

We developed a sequence-ready physical map of a part of human chromosome 12p12.3–p13.2 where the natural killer gene complex (NKC) is located. The NKC includes a cluster of genes with structure similar to that of the Ca2+-dependent lectin superfamily of glycoproteins that are expressed on the surface of most natural killer (NK) cells and a subset of T cells. These killer cell lectin-like receptors (KLR) are involved in NK target cell recognition, leading to activation or inhibition of NK cell function. We used a number of sequence-tagged site (STS) markers from this region to screen two large insert bacterial artificial chromosome (BAC) libraries and a bacteriophage P1-derived (PAC) chromosome library. The clones were assembled into contiguous sets by STS content analysis. The 72-BAC and 11-PAC contig covers nearly 2 Mb of DNA and provides an average marker resolution of 26 kb. We have precisely localized 17 genes, 5 expressed sequence tags, and 49 STSs within this contig. Of this total number of STS, 30 are newly developed by clone-end sequencing. We established the order of the genes as tel–M6PR–MAFAL (HGMW-approved symbol KLRG1)–A2M–PZP–A2MP–NKRP1A (HGMW-approved symbol KLRB1)–CD69–AICL (HGMW-approved symbol CLECSF2)–KLRF1–OLR1–CD94 (HGMW-approved symbol KLRD1)–NKG2D (HGMW-approved symbol D12S2489E)–PGFL–NKG2F (HGMW-approved symbol KLRC4)–NKG2E (HGMW-approved symbol KLRC3)–NKG2A (HGMW-approved symbol KLRC1)–LY49L (HGMW-approved symbol KLRA1)–cen. This map would facilitate the cloning of new KLR genes and the complete sequencing of this region.