Comparative analysis of the complete mitochondrial genomes of three Zeugodacus species (Insecta: Tephriti­dae: Dacinae) and their phylogenetic relationships with other congeners

• Published in: Arthropod systematics & phylogeny Vol. 81; no. 3; pp. 747 - 759
• Main Authors: Yong, Hoi-Sen, Song, Sze-Looi, Chua, Kah-Ooi, Liew, Yvonne Jing Mei, Suana, I. Wayan, Lim, Phaik-Eem, Chan, Kok-Gan, Eamsobhana, Praphathip
• Format: Journal Article
• Language: English
• Published: Dresden Pensoft Publishers 08-09-2023; Senckenberg Gesellschaft für Naturforschung
• Subjects: Amino acids; Analysis; Comparative analysis; Congeners; Fruit-flies; Gene order; Genes; Genetic testing; Genomes; Genomics; Javadacus; Mitochondrial DNA; Phylogenetics; Phylogeny; Sinodacus; Skewness; Stop codon; Transfer RNA; Zeugodacus; Malaysia; Indonesia
• ISSN: 1863-7221; 1864-8312
• DOI: 10.3897/asp.81.e105025

The complete mitogenomes of fruit flies Zeugodacus (Javadacus) calumniatus, Z. (Javadacus) heinrichi and Z. (Sinodacus) hochii have similar gene order and contain 37 genes and a non-coding region. They share an identical start codon for the respective protein-coding genes (PCGs), an identical TAA stop codon for 11 PCGs, TAG for
cob
, and an incomplete T stop codon for
nad5
. The cloverleaf structure of most of the tRNAs is similar in the three
Zeugodacus
species. Phylogenetic analyses reveal Z. (Parasinodacus) cilifer to be external to two main clades: (A) monophyletic subgenus Zeugodacus; and (B) subgenera
Javadacus
and
Sinodacus
. The present results indicate that the taxonomic status of some taxa needs clarification.
Z. calumniatus
is genetically very similar to
Z. tau
and is not congruent with its current placement in the
munda
complex.
Z. mukiae
NC_067083 is genetically very similar to
Z. scutellaris
, but differs significantly from
Z. mukiae
MG683384 of the
arisanicus
(
arisanica
) complex. On the other hand,
Z. proprediaphorus
is genetically distinct from and not a synonym of
Z. diaphorus
.
Z. caudatus
sensu stricto from Indonesia forms a sister lineage with
Z. diversus
, instead of with the Malaysian and Chinese taxa of
Z. caudatus
sensu lato. A notable incongruence is the sister lineage of Z. (Sinodacus) hochii and Z. (Javadacus) heinrichi among other taxa of subgenus Javadacus. A more extensive taxon sampling, particularly the subgenus Sinodacus (and other subgenera), is needed to clarify/resolve their subgenus status.