JUNO, the receptor of sperm IZUMO1, is expressed by the human oocyte and is essential for human fertilisation

• Published in: Human reproduction (Oxford) Vol. 34; no. 1; pp. 118 - 126
• Main Authors: Jean, C, Haghighirad, F, Zhu, Y, Chalbi, M, Ziyyat, A, Rubinstein, E, Gourier, C, Yip, P, Wolf, J P, Lee, J E, Boucheix, C, Barraud-Lange, V
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01-01-2019; Oxford University Press (OUP)
• Subjects: Animals; Antibodies, Monoclonal - pharmacology; Carrier Proteins - antagonists & inhibitors; Carrier Proteins - metabolism; Cell Culture Techniques; Cell Membrane - metabolism; Egg Proteins; Female; Fertilization - physiology; Fertilization in Vitro - methods; HEK293 Cells; Humans; Hybridomas; Immunoglobulins - metabolism; Life Sciences; Male; Membrane Proteins - metabolism; Mice; Oocytes - cytology; Oocytes - metabolism; Receptors, Cell Surface; Recombinant Proteins - metabolism; Sperm-Ovum Interactions - drug effects; Sperm-Ovum Interactions - physiology; Spermatozoa - metabolism; human gametes; oocyte; IZUMO1 protein; fertilisation; JUNO protein
• ISSN: 0268-1161; 1460-2350
• DOI: 10.1093/humrep/dey340

Abstract
STUDY QUESTION
Is JUNO protein present at the surface membrane of human oocytes and involved in the fertilisation process?
SUMMARY ANSWER
JUNO protein is expressed on the plasma membrane of human oocytes and its inhibition by a monoclonal antibody completely blocks gamete fusion.
WHAT IS KNOWN ALREADY
Fusion of gamete membranes is the culminating event of the fertilisation process, but its molecular mechanisms are poorly understood. Until now, three molecules have been shown to be essential: CD9 tetraspanin in the oocyte, Izumo1 protein on the sperm and Juno, its corresponding receptor on the oocyte. Oocyte CD9 and sperm IZUMO1 have been identified in human gametes and their interaction is also well-conserved among several mammalian species. The presence of JUNO on human oocytes, however, has not yet been reported, nor has its role in fertilisation been investigated.
STUDY DESIGN, SIZE, DURATION
We selected an anti-human JUNO antibody in order to investigate the presence of JUNO on the oocyte membrane surface and studied its potential involvement in gamete membrane interaction during fertilisation.
PARTICIPANTS/MATERIALS, SETTING, METHODS
Monoclonal antibodies against human JUNO (anti-hJUNO mAb) were produced by immunisation of mice with HEK cells transfected with the putative human JUNO sequence (HEK-hJUNO). These antibodies were used for immunostaining experiments and in vitro fertilisation assays with human gametes (GERMETHEQUE Biobank).
MAIN RESULTS AND THE ROLE OF CHANCE
Three hybridoma supernatants, verified by immunostaining, revealed specifically HEK-hJUNO cells. The three purified monoclonal antibodies, FJ2E4 (IgG1), FJ8E8 (IgG1) and FJ4F5 (IgG2a), recognised the soluble recombinant human JUNO protein and, in a western blot of HEK-hJUNO extracts, a protein with an expected MW of 25 kDa. In addition, soluble recombinant human IZUMO protein inhibited the binding of anti-hJUNO mAbs to cells expressing hJUNO. Using these anti-hJUNO mAbs in immunostaining, we identified the presence of JUNO protein at the plasma membrane of human oocytes. Furthermore, we revealed a progressive expression of JUNO according to oocyte maturity. Finally, we showed that human zona-free oocytes, inseminated in the presence of anti-hJUNO mAb, were not fertilised by human sperm. These results suggest that, as seen in the mouse, JUNO is indeed involved in human gamete membrane fusion during fertilisation.
LARGE-SCALE DATA
N/A.
LIMITATIONS, REASONS FOR CAUTION
In accordance with French bioethics laws, functional tests were performed using zona-free oocytes, which of course does not fully encompass all normal in vivo physiological conditions. However, these in vitro tests do provide direct information regarding sperm-oocyte membrane interactions.
WIDER IMPLICATIONS OF THE FINDINGS
Mechanisms of gamete fusion appear to be homologous between mice and humans. However, some differences do exist and analysing the human mechanisms is essential. In fact, this is the first report describing the presence of JUNO on human oocytes and its involvement in human fertilisation. This discovery allows further examination of the understanding of molecular mechanisms that drive gamete fusion: a crucial challenge at a time when infertility affects 16% of reproductively active couples.
STUDY FUNDING/COMPETING INTERESTS
This work was supported by the Agence Nationale pour la Recherche, Grant no. ANR-13-BVS5-0004, and by Association Institut du Cancer et d'Immunogénétique (ICIG). There are no competing interests.