Pediocin PA-1 and a pediocin producing Lactobacillus plantarum strain do not change the HMA rat microbiota

• Published in: International journal of food microbiology Vol. 130; no. 3; pp. 251 - 257
• Main Authors: Bernbom, Nete, Jelle, Birthe, Brogren, Carl-Henrik, Vogensen, Finn K., Nørrung, Birgit, Licht, Tine Rask
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-04-2009; [Amsterdam; New York, NY]: Elsevier Science
• Subjects: animal models; Animals; antibacterial properties; Bacteriocins - pharmacology; commensalism; DGGE; feces; food biopreservatives; Food Preservatives; Gastrointestinal Tract - microbiology; Human Microbiota Associated rats; Humans; intestinal microorganisms; lactic acid bacteria; Lactobacillus plantarum; Lactobacillus plantarum - metabolism; Pediocin PA-1; Pediocins; Rats; Rats, Sprague-Dawley; selective media; Lactobacillus plantarum; Pediocin PA-1; DGGE; Human Microbiota Associated rats
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2009.02.003

The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal gut microbiota is important for assessment of consumer risks associated with the use of biopreservative cultures. Effects of ingested (i) pediocin PA-1 producing
Lactobacillus plantarum DDEN 11007, (ii) the plasmid cured pediocin negative
L. plantarum DDEN 12305, or (iii) supernatants of either of these two strains on the composition of the intestinal microbiota of Human Microbiota Associated (HMA) rats were examined by selective cultivation and molecular methods. The culturable microbiota was in all treatments dominated by lactic acid bacteria and coliforms and no changes in the rat commensal microbiota were detected after ingestion of either of the two
L. plantarum strains as determined by both culturable methods and molecular methods (DGGE). Both strains were detected in the faeces after ingestion. Pediocin PA-1 did not mediate changes of the rat microbiota, and a biological assay indicated that the bacteriocin was degraded or inactivated during passage through the intestine.