Nucleotide Sequences of All the γ Gene Loci of Murine Immunoglobulin Heavy Chains

• Published in: Genomics (San Diego, Calif.) Vol. 41; no. 1; pp. 100 - 104
• Main Authors: Akahori, Y., Kurosawa, Y.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-04-1997; Elsevier
• Subjects: Animals; Base Sequence; Biological and medical sciences; Chromosome Mapping; Consensus Sequence; Conserved Sequence; DNA Primers - genetics; Fundamental and applied biological sciences. Psychology; Genes, Immunoglobulin; Genes. Genome; Humans; Immunoglobulin gamma-Chains - genetics; Mice; Models, Genetic; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; Pseudogenes; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Sequence Homology, Nucleic Acid; Species Specificity; Pseudogene; Heavy peptide chain; Immunoglobulins; Genome organization; Repeated sequence; 3' terminal-Sequence; Rodentia; Gene organization; Molecular evolution; Vertebrata; Immunogenetics; Conserved sequence; Mammalia; Mouse; Genetics
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1997.4606

The nucleotide sequence of the entire region that encodes the γ chains of mouse immunoglobulin was determined, namely, 11,942 bp that include the whole γ3 gene and 59,647 bp from the γ1 to the γ2a gene. The sequence of 21,012 bp that includes the entire human γ3 gene was also determined. The present analysis revealed three new findings. (1) The S regions are composed of repetitive sequences. A 26-bp repeating unit, in addition to a 49-bp repeating unit, is present in the Sγ2b and Sγ2a regions. In the case of the γ2b gene, the 26-bp unit is repeated 5 times and the 49-bp unit is repeated 69 times. In the case of the γ2a gene, by contrast, the 26-bp unit is repeated 63 times and the 49-bp unit is repeated 14 times. (2) Two pseudo-γ-genes were identified between the γ1 and the γ2b genes as well as between the γ2b genes and the γ2a genes. (3) A possible functional region was identified in the region downstream of each γ gene. This region, designated the 3′ conserved region (3′CR), was also found in the region downstream of the human γ3 gene. Duplication of a primordial γ2 gene seems likely to have occurred by homologous recombination that involved the 3′CR sequences.