A turn-on fluorescent GFP chromophore analog for highly selective and efficient detection of H2S in aqueous and in living cells

• Published in: Sensors and actuators. B, Chemical Vol. 298; p. 126875
• Main Authors: Mittapelli, Lavanya L., Nawale, Ganesh N., Gholap, Sachin P., Varghese, Oommen P., Gore, Kiran R.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01-11-2019; Elsevier Science Ltd
• Subjects: Cancer; Chemosensor; Chromophores; Colon; Cysteine; Fibroblasts; Fluorescence; Green fluorescent protein; H2S detection; Hydrogen sulfide; Live cell imaging; Neurotransmitters; Physiology; Selectivity; Thiols; Chemosensor; Cysteine; H2S detection; Green fluorescent protein; Live cell imaging
• ISSN: 0925-4005; 1873-3077; 1873-3077
• DOI: 10.1016/j.snb.2019.126875

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•Novel synthetic acryloyl protected GFP chromophore based H2S chemodosimeter.•Rapid, selective and sensitive detection of H2S over wide range of anions and competitive biological thiols.•Efficient to detect H2S in live cells such as HCT and HDF cells.•Exhibited around 290-folds fluorescence enhancement and 1000 times lower detection limit compared to reported WHO limit.
Hydrogen sulphide is a gaseous neurotransmitter responsible for neuronal function and controls vast range of physiological functions. Herein, we report the synthesis and evaluation of novel Green Fluorescent Protein (GFP) chromophore analog, acryloyl-4-(p-hydroxybenzylidene)-5-imidazolidinone (AHBI) for turn-on fluorescent detection of H2S over wide range of anions and various biologically important competitive thiols. AHBI probe exhibited high selectivity and sensitivity, high fluorescence stability, large stokes shift and lower detection limit (15.85 ppb) for H2S in complete water medium. Cell imaging studies in human colon cancer cells (HCT116) and normal human dermal fibroblasts (HDF) confirmed the compatibility and versatility of AHBI probe at micromolar level. Overall, we believe the AHBI, as an optical probe will be useful to investigate the role of H2S in various physiological processes, regulation of cancer cell growth, and in pathogenic events.