Effect of Dehydroepiandrosterone Sulfate on Maturation and Functional Properties of Interferon-α-Induced Dendritic Cells

• Published in: Bulletin of experimental biology and medicine Vol. 148; no. 1; pp. 68 - 71
• Main Authors: Leplina, O. Yu, Tikhonova, M. A, Sakchno, L. V, Tyrinova, T. V, Ostanin, A. A, Chernykh, E. R
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01-07-2009; Springer US
• Subjects: Antigens, CD - immunology; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell Differentiation; cytokines; Dehydroepiandrosterone Sulfate - pharmacology; dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - immunology; Humans; Interferon-alpha - pharmacology; Internal Medicine; Laboratory Medicine; Pathology; phenotype; prasterone; phenotype; dehydroepiandrosterone sulfate; cytokines; dendritic cells
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-009-0619-7

We studied the effect of adrenal cortex hormone dehydroepiandrosterone sulfate on maturation and functional activity of interferon-α-induced dendritic cells. Dehydroepiandrosterone sulfate stimulated differentiation and maturation of interferon-α-induced dendritic cell, which manifested in a decrease in the number of CD14⁺ cells and increase in the ratio of mature CD83⁺ dendritic cells expressing costimulatory molecules (CD80 and CD86). The induction of dendritic cell differentiation after treatment with dehydroepiandrosterone sulfate was accompanied by an increase in the production of interferon-γ. At the stage of dendritic cell maturation, the effect of dehydroepiandrosterone sulfate manifested in a 4-fold increase in tumor necrosis factor-α production. Dehydroepiandrosterone sulfate had little effect on the production of Th2/antiinfl ammatory cytokines at the stages of differentiation and maturation of interferon-α-induced dendritic cells. Dehydroepiandrosterone sulfate increased the ability of dendritic cells to stimulate Th1 cytokine production by T cells (interferon-γ). This hormone had no effect on the ability of interferon-α-induced dendritic cells to activate CD3⁺IL-4⁺T cells in mixed lymphocyte culture.