Enzyme inhibitory and antioxidant activities of Viburnum tinus L. relevant to its neuroprotective potential

• Published in: Food chemistry Vol. 141; no. 1; pp. 582 - 588
• Main Authors: Yılmaz, Betül Sever, Altun, Mehmet Levent, Orhan, Ilkay Erdogan, Ergene, Burcin, Citoglu, Gülcin Saltan
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-11-2013; Elsevier
• Subjects: Acetylcholinesterase - analysis; Agaricales; Animals; Antioxidant activity; Antioxidants - chemistry; Biological and medical sciences; Butyrylcholinesterase - analysis; Cholinesterase Inhibitors - chemistry; Electrophorus; Enzyme inhibition; Enzyme Inhibitors - chemistry; Food toxicology; Fruit - chemistry; Horses; Medical sciences; Monophenol Monooxygenase - analysis; Monophenol Monooxygenase - antagonists & inhibitors; Neuroprotection; Neuroprotective Agents - chemistry; Plant Extracts - chemistry; Radical-scavenging; Toxicology; Viburnum - chemistry; Viburnum tinus; Enzyme inhibition; Neuroprotection; Radical-scavenging; Viburnum tinus; Antioxidant activity; Enzyme; Neuroprotective agent; Inhibition; Antioxidant; Radical scavenger
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.03.020

•Neurodegeneration-related enzyme inhibitory effects of Viburnum tinus was tested.•Antioxidant activity of the plant was tested in seven in vitro assays.•The branch–ethyl acetate and fruit–methanol extract had significant anticholinesterase effect.•Tyrosinase inhibitory activity of V. tinus was examined for the first time.•This is the first study on cholinesterase inhibition of V. tinus.
In vitro neuroprotective activity of the extracts of Viburnum tinus L. was investigated via inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and tyrosinase (TYRO) by microtitre plate assays. Their antioxidant activity was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD), super oxide (SO), and nitric oxide (NO) radical-scavenging activities, ferric ion-chelation capacity, ferric- (FRAP), and phosphomolybdenum-reducing antioxidant power (PRAP) assays. Total phenol and flavonoid content of the extracts was determined spectrophotometrically. The branch–ethyl acetate and fruit–methanol extracts exerted potent anticholinesterase effects (66.4±0.65% to 97.7±0.47%), while the fruit–methanol extract had the highest TYRO inhibition (47.0±0.68%). The methanol extracts showed higher activities in most of the antioxidant tests. All the extracts displayed notable NO-scavenging effects (47.5±5.03% to 74.5±1.80%). Only the fruit–ethyl acetate extract quenched SO radical (38.4±1.01%) at 500μgml−1. Our data indicate that the fruit and branch extracts of V. tinus may provide potential neuroprotection.