Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and Guidance

• Published in: Cerebral cortex (New York, N.Y. 1991) Vol. 25; no. 11; pp. 4111 - 4125
• Main Authors: Mita, Sakura, de Monasterio-Schrader, Patricia, Fünfschilling, Ursula, Kawasaki, Takahiko, Mizuno, Hidenobu, Iwasato, Takuji, Nave, Klaus-Armin, Werner, Hauke B, Hirata, Tatsumi
• Format: Journal Article
• Language: English
• Published: United States 01-11-2015
• Subjects: Age Factors; Animals; Animals, Newborn; Cells, Cultured; Cerebral Cortex - cytology; Cerebral Cortex - physiology; Corpus Callosum - cytology; Corpus Callosum - physiology; Embryo, Mammalian; Female; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Homeodomain Proteins - metabolism; In Vitro Techniques; Male; Membrane Glycoproteins - deficiency; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Myelin Proteolipid Protein - deficiency; Myelin Proteolipid Protein - genetics; Nerve Tissue Proteins - deficiency; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Neurites - physiology; Neurons - cytology; Nuclear Proteins - metabolism; Repressor Proteins - metabolism; Tumor Suppressor Proteins - metabolism; Gpm6a; Plp1; corpus callosum; axon pathfinding; Gpm6b
• ISSN: 1047-3211; 1460-2199
• DOI: 10.1093/cercor/bhu129

The function of mature neurons critically relies on the developmental outgrowth and projection of their cellular processes. It has long been postulated that the neuronal glycoproteins M6a and M6b are involved in axon growth because these four-transmembrane domain-proteins of the proteolipid protein family are highly enriched on growth cones, but in vivo evidence has been lacking. Here, we report that the function of M6 proteins is required for normal axonal extension and guidance in vivo. In mice lacking both M6a and M6b, a severe hypoplasia of axon tracts was manifested. Most strikingly, the corpus callosum was reduced in thickness despite normal densities of cortical projection neurons. In single neuron tracing, many axons appeared shorter and disorganized in the double-mutant cortex, and some of them were even misdirected laterally toward the subcortex. Probst bundles were not observed. Upon culturing, double-mutant cortical and cerebellar neurons displayed impaired neurite outgrowth, indicating a cell-intrinsic function of M6 proteins. A rescue experiment showed that the intracellular loop of M6a is essential for the support of neurite extension. We propose that M6 proteins are required for proper extension and guidance of callosal axons that follow one of the most complex trajectories in the mammalian nervous system.