Stimulations inducing tethered lipid bilayer formation on protein‐modified substrate

Supported lipid bilayer (SLB) is one artificial biomembrane system used to study transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is the transformation of spherical lipid vesicles to a planar membrane. We investigated s...

Full description

Saved in:

Bibliographic Details
Published in:	Electronics and communications in Japan Vol. 103; no. 9; pp. 36 - 42
Main Authors:	Tero, Ryugo, Sato, Junichi
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc 01-09-2020
Subjects:	Calcium ions fluorescence microscopy lipid bilayer membrane Lipids Silicon dioxide Silicon substrates surface chemical modification vesicle fusion Vesicles
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Supported lipid bilayer (SLB) is one artificial biomembrane system used to study transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is the transformation of spherical lipid vesicles to a planar membrane. We investigated stimulation methods for adsorbed lipid vesicles on a streptavidin‐modified SiO2/Si substrate to induce tethered‐type SLB. We describe the effects of osmotic stress, Ca2+ addition, and a freeze–thaw cycle on the adsorbed vesicles, and on the condition of t‐SLB.
Bibliography:	Denki Gakkai Ronbunshi C Translated from Volume 140 Number 4, pages 447–451, DOI IEEJ Transactions on Electronics, Information and Systems of 10.1541/ieejeiss.140.447
ISSN:	1942-9533 1942-9541
DOI:	10.1002/ecj.12262