Interleukin-1 and tumor necrosis factor-α gene polymorphisms in Turkish patients with localized aggressive periodontitis

• Published in: Journal of Oral Science Vol. 50; no. 2; pp. 151 - 159
• Main Authors: Guzeldemir, Esra, Gunhan, Meral, Ozcelik, Onur, Tastan, Hakki
• Format: Journal Article
• Language: English
• Published: Japan Nihon University School of Dentistry 01-06-2008
• Subjects: Adolescent; Adult; Alleles; Dentistry; Electrophoresis, Agar Gel; Female; genetic polymorphisms; Genetic Predisposition to Disease - genetics; Genotype; Heterozygote; Homozygote; Humans; Inflammation Mediators; Interleukin-1 - genetics; Interleukin-1alpha - genetics; Interleukin-1beta - genetics; interleukin-1α; interleukin-1β; localized aggressive periodontitis; Male; Periodontitis - genetics; Periodontitis - immunology; Polymerase Chain Reaction; Polymorphism, Genetic - genetics; Restriction Mapping; Risk Factors; Tumor Necrosis Factor-alpha - genetics; tumor necrosis factor-α; Turkey; Turkey
• ISSN: 1343-4934; 1880-4926
• DOI: 10.2334/josnusd.50.151

Localized aggressive periodontitis (LAgP) is a complex multifactorial periodontal disease to which genetic factors are thought to predispose individuals. Interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) are potent immunomodulators and proinflammatory cytokines that have been implicated in the pathogenesis of autoimmune and infectious diseases and proposed to be risk factors for LAgP. Our aim was to investigate IL-1 α +4845, IL-1 β +3954, and TNF-α -308 gene polymorphisms in Turkish LAgP patients. We genotyped 31 LAgP patients and 31 healthy controls for IL-1α+4845, IL-1β+3954, and TNF-α-308 using standard PCR amplification followed by restriction enzyme digestion and gel electrophoresis. Higher prevalence of heterozygosity for IL-1α+4845 was found in cases (65%) when compared to controls (35%) (P < 0.05). While homozygous allele 1 of IL-1β+3954 was the most frequent genotype in cases (62%), no controls were homozygous for this allele (P < 0.001). Homozygous allele 1 was the most common TNF-α genotype in both groups, however no significant difference in TNF-α genotypes was found between groups. In conclusion, in this Turkish population, susceptibility to LAgP is increased by heterozygosity for allele 1 of IL-1α+4845 or homozygosity for allele 1 of IL-1β+3954. Moreover, IL-1 gene polymorphisms appear to have a role in susceptibility to LAgP, and the above-mentioned genotypes could be an important risk factor for LAgP in the Turkish population. (J. Oral Sci. 50, 151-159, 2008)