Ecophysiological responses of juvenile seabass (Dicentrarchus labrax) exposed to increased temperature and dietary methylmercury

• Published in: The Science of the total environment Vol. 586; pp. 551 - 558
• Main Authors: Maulvault, Ana Luísa, Barbosa, Vera, Alves, Ricardo, Custódio, Ana, Anacleto, Patrícia, Repolho, Tiago, Pousão Ferreira, Pedro, Rosa, Rui, Marques, António, Diniz, Mário
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-05-2017
• Subjects: Animal condition; Animals; Bass - physiology; Climate Change; Dietary MeHg; Heat shock; Methylmercury Compounds - adverse effects; Neurotoxicity; Oxidative stress; Seawater - chemistry; Seawater warming; Temperature; Water Pollutants, Chemical - adverse effects; Dietary MeHg; Oxidative stress; Animal condition; Neurotoxicity; Seawater warming; Heat shock
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2017.02.016

The ecotoxicological effects of methylmercury (MeHg) exposure have been intensively described in literature. Yet, it is still unclear how marine biota will respond to the presence of MeHg under climate change, namely ocean warming. The present study aimed to investigate, for the first time, fish condition [Fulton's K index (K), hepatosomatic index (HIS) and brain-to-body mass ratio (BB-ratio)] and several stress-related responses in an ecologically and commercially important fish species (Dicentrachus labrax) exposed for 28days to dietary MeHg (8.0mg kg-1 dw) and temperature increase (+4°C). Results showed significant impairments on fish condition, i.e. up to 34% decrease on K, >100% increase on HIS and 44% decrease on BB-ratio, compared to control conditions. Significant changes on tissue biochemical responses were observed in fish exposed to both stressors, acting alone or combined, evidencing the relevance of assessing possible interactions between different environmental stressors in ecotoxicological studies. For instance, muscle showed to be the least affected tissue, only revealing significant alterations in GST activity of MeHg-enriched fish. On the other hand, liver exhibited a significant induction of GST (>100%) and CAT (up to 74%) in MeHg-enriched fish, regardless of temperature exposure, as well as decreased SOD activity (19%) and increased HSP70/HSC70 content (87%) in fish exposed to warming alone. Brain showed to be affected by temperature (69% of GST inhibition and >100% of increased CAT activity), MeHg (>100% of increased CAT activity, 47% of SOD inhibition and 55% of AChE inhibition), as well as by the combination of both (GST, SOD and AChE inhibition, 17%, 48% and 53%, respectively). Hence, our data provides evidences that the toxicological aspects of MeHg ca be potentiated by warmer temperatures, thus, evidencing the need for further research combining contaminants exposure and climate change effects, to better forecast ecological impacts in the ocean of tomorrow.
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•MeHg exposure decreased K and BB-ratio, but increased HSI•Muscle GST was induced by warming and MeHg, but not by their combination•Brain CAT was induced by warming and MeHg, but not by their combination•Liver GST and CAT were induced by MeHg, regardless of temperature exposure•MeHg exposure did not enhance HSP70/HSC70 induction caused by warming•Increased temperature did not enhance AChE inhibition due to MeHg exposure