Effects of chronic exposure to microplastics of different polymer types on early life stages of sea trout Salmo trutta

• Published in: The Science of the total environment Vol. 740; p. 139922
• Main Authors: Jakubowska, Magdalena, Białowąs, Marcin, Stankevičiūtė, Milda, Chomiczewska, Agnieszka, Pažusienė, Janina, Jonko-Sobuś, Karolina, Hallmann, Anna, Urban-Malinga, Barbara
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 20-10-2020
• Subjects: Corticosteroids; Fish embryos; Fish larvae; Genotoxicity; Growth rate; Non-ingestion related exposure; Fish larvae; Corticosteroids; Growth rate; Fish embryos; Non-ingestion related exposure; Genotoxicity
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2020.139922

The aim of the present study was to determine the effect of a long-term (113 days) exposure to microplastics on the development and induction of endocrine, geno- and cytotoxic responses in early life stages of sea trout Salmo trutta. Microplastic particles (3000 μm) of three most commonly mass-produced polymers (polystyrene - PS, polyethylene terephthalate - PET and polyethylene - PE) were applied in environmentally realistic concentrations (0.1% of sediment dry weight) in a laboratory experiment imitating the natural environment, typical for sea trout spawning grounds. The exposure of the sea trout, from fertilized eggs to mobile yolk-sac larvae, to microplastics did not affect the hatching success (the survival of embryos), hatching rate and the incubation period. Microplastics of any tested polymer type also had no adverse effect on the larvae survival, growth rate and the rate of yolk sack absorption. Similarly, no changes in frequencies of detected cytotoxicity endpoints compared to the control group were recorded. Exposure to polymer particles induced however the formation of genotoxicity endpoints (nuclear buds, micronuclei and blebbed nuclei cells). The level of total genotoxicity (ΣGentox) in fish larvae erythrocytes increased significantly in the following sequence: PS > PET > PE. No significant changes in the whole body corticosterone, dehydrocorticosterone and cortisone concentrations due to exposure to microplastics were recorded, while cortisol was detected in larvae exposed to PS. Our results show that long-term, non-ingestion related exposure to microplastics does not affect development of S. trutta early life stages but may lead to genotoxic responses. PS seems to be the most hazardous among all polymers studied. This is the first study demonstrating non-ingestion related toxicity of microplastics to the early life stages of fish.
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•Effects of microplastics (MPs) on sea trout embryos and larvae were assessed.•Exposure to MPs of any polymer type did not affect embryos hatching success or rate.•Larvae survival, growth and frequency of cytotoxic endpoints were not affected.•Larvae exhibited increased frequency of genotoxic endpoints after exposure to MPs.•Cortisol was detected in larvae exposed to polystyrene.