Disappearance of Quorum Sensing in Burkholderia glumae During Experimental Evolution

• Published in: Microbial ecology Vol. 79; no. 4; pp. 947 - 959
• Main Authors: Gnanasekaran, Gopalsamy, Lim, Jae Yun, Hwang, Ingyu
• Format: Journal Article
• Language: English
• Published: New York Springer Science + Business Media 01-05-2020; Springer US; Springer Nature B.V
• Subjects: Adaptation; Biomedical and Life Sciences; Burkholderia glumae; Catalase; Cell density; Clones; Cloning; Detection; Ecology; Evolution; Failure analysis; Gene expression; Genes; GENES AND GENOMES; Geoecology/Natural Processes; Laboratories; Life Sciences; Microbial Ecology; Microbiological strains; Microbiology; Mutation; Nature Conservation; Oxalic acid; Oxidative stress; Parameter estimation; Pathogens; Phytotoxins; Quorum sensing; Sequence analysis; Sequencing; Signaling; Social behavior; Stationary phase; Subculture; Water Quality/Water Pollution; Autoinducer; Quorum sensing; Experimental evolution; Plant pathogen; Burkholderia
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-019-01445-0

The plant pathogen Burkholderia glumae uses quorum sensing (QS) that allows bacteria to share information and alter gene expression on the basis of cell density. The wild-type strain of B. glumae produces quorum-sensing signals (autoinducers) to detect their community and upregulate QS-dependent genes across the population for performing social and group behaviors. The model organism B. glumae was selected to investigate adaptation, estimate evolutionary parameters, and test diverse evolutionary hypotheses by using experimental evolution. The wild-type B. glumae virulent strain showed genotypic changes during regular subculture due to oxygen limitation. The laboratory-evolved clones failed to produce the signaling molecule of C8-HSL/C6-HSL for activation of the quorum-sensing system. Further, the laboratory-evolved clones failed to produce catalase and oxalate for protecting themselves from the toxic environment at stationary phase and phytotoxins (toxoflavin) for infecting rice grain, respectively. The laboratory-evolved clones were completely sequenced and compared with the wild-type. Sequencing analysis of the evolved clones revealed that mutations in QS-responsible genes (iclR), sensor genes (shk, mcp), and signaling genes (luxR) were responsible for quorum-sensing activity failure. The experimental results and sequencing analysis revealed quorum-sensing process failure in the laboratory-evolved clones. In conclusion, the wild-type B. glumae strain was often exposed to oxidative stress during regular subculture and evolved as an avirulent strain (quorum-sensing mutant) by losing the phenotypic and genotypic characteristics.