A survey of 16S rRNA and amoA genes related to autotrophic ammonia-oxidizing bacteria of the beta-subdivision of the class proteobacteria in contaminated groundwater

• Published in: Canadian journal of microbiology Vol. 46; no. 11; p. 1012
• Main Authors: Ivanova, I A, Stephen, J R, Chang, Y J, Brüggemann, J, Long, P E, McKinley, J P, Kowalchuk, G A, White, D C, Macnaughton, S J
• Format: Journal Article
• Language: English
• Published: Canada 01-11-2000
• Subjects: Ammonia - metabolism; Bacterial Proteins - genetics; Betaproteobacteria - classification; Betaproteobacteria - genetics; Betaproteobacteria - metabolism; Biodegradation, Environmental; Ecology; Genes, Bacterial; Genetic Variation; Geological Phenomena; Geology; Mining - legislation & jurisprudence; New Mexico; Nitrosomonas - classification; Nitrosomonas - genetics; Nitrosomonas - metabolism; Oxidoreductases - genetics; Phylogeny; RNA, Ribosomal, 16S - genetics; Uranium; Water Microbiology; Water Pollutants, Chemical - metabolism; New Mexico
• ISSN: 0008-4166
• DOI: 10.1139/cjm-46-11-1012

In this study, we investigated the size and structure of autotrophic ammonia oxidizer (AAO) communities in the groundwater of a contamination plume originating from a mill-tailings disposal site. The site has high levels of dissolved N from anthropogenic sources, and exhibited wide variations in the concentrations of NO3- and NH3 + NH4+. Community structures were examined by PCR-DGGE targeting 16S rDNA with band excision and sequence analysis, and by analysis of amoA fragment clone libraries. AAO population sizes were estimated by competitive PCR targeting the gene amoA, and correlated significantly with nitrate concentration. Most samples revealed novel diversity in AAO 16S rDNA and amoA gene sequences. Both 16S rDNA and amoA analyses suggested that all samples were dominated by Nitrosomonas sp., Nitrosospira sp. being detected in only 3 of 15 samples. This study indicated numerical dominance of Nitrosomonas over Nitrosospira in groundwater, and suggests that groundwater ammonia oxidizers are more similar to those dominating freshwater sediments than bulk soil.