Expression of DREB-Like Genes in Coffea canephora and C. arabica Subjected to Various Types of Abiotic Stress

• Published in: Tropical plant biology Vol. 12; no. 2; pp. 98 - 116
• Main Authors: Torres, Luana Ferreira, Reichel, Tharyn, Déchamp, Eveline, de Aquino, Sinara Oliveira, Duarte, Karoline Estefani, Alves, Gabriel Sergio Costa, Silva, Anderson Tadeu, Cotta, Michelle Guitton, Costa, Tatiana Santos, Diniz, Leandro Eugenio Cardamone, Breitler, Jean-Christophe, Collin, Myriam, Paiva, Luciano Vilela, Andrade, Alan Carvalho, Etienne, Hervé, Marraccini, Pierre
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-06-2019; Springer Nature B.V
• Subjects: Abiotic stress; Abscisic acid; Biomedical and Life Sciences; Coffea arabica; Coffea canephora; Coffee; Cold; Drought; Drought resistance; Gene expression; Gene regulation; Genes; Guard cells; Haplotypes; High temperature; Humidity; Leaves; Life Sciences; Low temperature; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant Sciences; Relative humidity; Roots; Transgenics; Vegetal Biology; Abiotic stress; gene expression - promoter; Abscisic acid - coffee; Abscisic acid; promoter; DREB; gene expression; coffee
• ISSN: 1935-9756; 1935-9764; 1935-9756
• DOI: 10.1007/s12042-019-09223-5

The aim of this work was to study the regulation of coffee
DREB
-like genes in leaves of
C. arabica
subjected to cold, heat, low relative humidity, exogenous abscisic acid and high light stress, as well as in leaves and roots of drought-tolerant and drought-susceptible clones of
Coffea canephora
subjected to water limitation. In
C. arabica
,
CaERF017
was the most expressed gene under low temperatures and relative humidity
,
while low humidity and high temperatures up-regulated the expression of
CaERF053
and
CaERF014
, respectively. Under water limitation,
CcDREB1B
,
CcRAP2.4
,
CcERF027
,
CcDREB1D
and
CcTINY
were the most expressed genes mainly in leaves of drought-tolerant
C. canephora
. On the other hand, expression of the
CcERF016
,
CcRAP2.4
and
CcDREB2F
genes was highly up-regulated under water limitation in the roots of drought-susceptible
C. canephora
clone 22
.
We previously reported fine-tuned regulation of
CcDREB1D
promoter haplotypes (HP15, HP16 and HP17) in transgenic
C. arabica
subjected to low humidity. Here, we investigated the regulation of these haplotypes under high light, cold, heat, and abscisic acid (ABA) stress. In apical buds and leaf guard cells, GUS-stained percentages were higher in pHP16L-transformed plants subjected to low humidity, high light and ABA stress than in pHP17L- and pHP15L-transformed plants. We also reported up-regulated expression of the endogenous
CaDREB1D
gene for both the cold and low humidity in leaves of pHP16L-transformed
C. arabica
suggesting a key role of this gene in controlling the responses of coffee plants to abiotic stress probably through an ABA-dependent pathway.