Enhanced osteogenic differentiation of mesenchymal stem cells on metal–organic framework based on copper, zinc, and imidazole coated poly‐l‐lactic acid nanofiber scaffolds

• Published in: Journal of biomedical materials research. Part A Vol. 107; no. 8; pp. 1841 - 1848
• Main Authors: Telgerd, M. D., Sadeghinia, Mohammad, Birhanu, Gebremariam, Daryasari, M. P., Zandi‐Karimi, Ali, Sadeghinia, Ali, Akbarijavar, Hamid, Karami, M. H., Seyedjafari, Ehsan
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-08-2019; Wiley Subscription Services, Inc
• Subjects: Adipose tissue; Angiogenesis; Biocompatibility; Biological activity; Cell culture; Coatings; Copper; Differentiation (biology); Imidazole; Lactic acid; Mesenchymal stem cells; Mesenchyme; Metal-organic frameworks; Metals; metal–organic framework; Minerals; nanofiber scaffolds; Nanofibers; Nanoparticles; Osteoconduction; Osteogenesis; Polylactic acid; Polymers; poly‐l‐lactic acid; Scaffolds; Stem cells; Tissue culture; Tissue engineering; Zinc; Zinc coatings; tissue engineering; nanofiber scaffolds; mesenchymal stem cells; poly-l-lactic acid; copper; zinc; metal-organic framework
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.36707

The presence of inorganic bioactive minerals with polymers can accelerate and promote several processes including: bone cell joining, proliferation, differentiation, and expression of osteogenic proteins. In this study, zinc (Zn), copper (Cu), and imidazole metal–organic framework (MOF) nanoparticles were synthesized and coated over poly‐l‐lactic acid (PLLA) nanofibrous scaffolds for bone tissue engineering application. The surface and bioactive features of the scaffolds were characterized. The osteogenic potential of the scaffolds on human adipose tissue‐derived mesenchymal stem cells (MSCs) was evaluated. Zn–Cu imidazole MOF coated PLLA scaffolds (PLLA@MOF) showed a comparable rate of MSC proliferation with the pure PLLA scaffolds and tissue culture plate (TCP). However, the PLLA@MOF potential of osteogenic differentiation was significantly greater than either pristine PLLA scaffolds or TCP. Hence, coating Zn–Cu imidazole MOF has a significant effect on the osteogenesis of MSC. Therefore, PLLA@MOF is novel scaffolds with bioactive components which are crucial for osteoconductivity and also able to provoke the osteogenesis and angiogenesis.