Nanotextured titanium surfaces stimulate spreading, migration, and growth of rat mast cells

• Published in: Journal of biomedical materials research. Part A Vol. 105; no. 8; pp. 2150 - 2161
• Main Authors: Marcatti Amarú Maximiano, William, Marino Mazucato, Vivian, Tambasco de Oliveira, Paulo, Célia Jamur, Maria, Oliver, Constance
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-08-2017
• Subjects: Adherent cells; Animals; Biocompatibility; Biocompatible Materials - chemistry; biomaterial; Cell Adhesion; Cell culture; Cell Line; Cell migration; Cell Movement; Cell Proliferation; Cytoskeleton; Dental prosthetics; Histamine; Immune system; Inflammation; Inflammatory response; mast cell; Mast cells; Mast Cells - cytology; migration; morphology; Nanostructure; Nanostructures - chemistry; nanotopography; Physiology; Rats; Surface chemistry; Surface Properties; Surgical implants; Titanium; Titanium - chemistry; Titanium base alloys; Transplants & implants; mast cell; migration; nanotopography; biomaterial; morphology; titanium
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.36076

Titanium is a biomaterial widely used in dental and orthopedic implants. Since tissue–implant interactions occur at the nanoscale level, nanotextured titanium surfaces may affect cellular activity and modulate the tissue response that occurs at the tissue–implant interface. Therefore, the characterization of diverse cell types in response to titanium surfaces with nanotopography is important for the rational design of implants. Mast cells are multifunctional cells of the immune system that release a range of chemical mediators involved in the inflammatory response that occurs at the tissue–implant interface. Therefore, the aim of this study was to investigate the effects of the nanotopography of titanium surfaces on the physiology of mast cells. The results show that the nanotopography of titanium surfaces promoted the spreading of mast cells, which was accompanied by the reorganization of the cytoskeleton. Also, the nanotopography of titanium surfaces enhanced cell migration and cell growth, but did not alter the number of adherent cells in first hours of culture or affect focal adhesions and mediator release. Thus, the results show that nanotopography of titanium surfaces can affect mast cell physiology, and represents an improved strategy for the rational production of surfaces that stimulate tissue integration with the titanium implants. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2150–2161, 2017.