TSOL18/HP6-Tsol, an immunogenic Taenia solium oncospheral adhesion protein and potential protective antigen

• Published in: Parasitology research (1987) Vol. 102; no. 5; pp. 921 - 926
• Main Authors: Parkhouse, R. Michael E, Bonay, Pedro, González, Luis Miguel, Ferrer, Elizabeth, Gárate, Teresa, Aguilar, Cruz M, Cortez A., Milagros M, Harrison, Leslie J. S
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-04-2008; Springer-Verlag; Springer
• Subjects: Animals; Antibodies, Helminth - biosynthesis; Antigens, Helminth - chemistry; Antigens, Helminth - genetics; Antigens, Helminth - immunology; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cell Adhesion; Cercopithecus aethiops; COS Cells; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Humans; Immunology; Invertebrates; Kidney - cytology; Medical Microbiology; Microbiology; Molecular Sequence Data; Original Paper; Rats; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Recombinant Fusion Proteins - metabolism; RNA, Messenger; Sequence Analysis, DNA; Taenia solium - chemistry; Taenia solium - genetics; Taenia solium - immunology; Taenia solium - pathogenicity; Tissue Invasion; Lasergene Software; Cell Adhesion Assay; Praziquantel; Fibronectin Type; Antigen; Helmintha; Plathelmintha; Parasite; Cestoda; Invertebrata; Adhesion; Taenia solium; Protein
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-007-0853-8

In this study, we employed Taenia solium mRNA extracted from a tapeworm of Venezuelan origin to clone express and test the recombinant protein of the T. solium homologue of the 18-kDa oncospheral adhesion molecule of Taenia saginata (HP6-Tsag/TSA18). We first confirm the conserved nature of the sequence of the T. solium homologue (TSOL18/HP6-Tsol) and demonstrate that the recombinant protein, which, as with its T. saginata homologue, is characterised by a fibronectin type III homology region, functions as an adhesion molecule. This emphasises the possible importance of TSOL18/HP6-Tsol in tissue invasion, thus providing a rational explanation for its efficacy as a vaccine. As protection against Taenia spp., oncospheres is antibody mediated, logically, therefore, TSOL18/HP6-Tsol may also serve as a diagnostic antigen, as is indeed the case for recombinant HP6-Tsag/TSA18.