A new method of estimating thermal performance of embryonic development rate yields accurate prediction of embryonic age in wild reptile nests

• Published in: Journal of thermal biology Vol. 74; pp. 187 - 194
• Main Authors: Rollinson, Njal, Holt, Sarah M., Massey, Melanie D., Holt, Richard C., Nancekivell, E. Graham, Brooks, Ronald J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-05-2018; Elsevier BV
• Subjects: Age; Chelydra serpentina; Developmental stages; Embryogenesis; Embryos; Fluctuating temperature; Heat units; Incubation; Information theory; Nests; Reptile; Reptiles & amphibians; Sex determination; Temperature; Temperature effects; Thermal performance; Turtle; Turtle; Reptile; Incubation; Heat units; Sex determination; Fluctuating temperature; Thermal performance
• ISSN: 0306-4565; 1879-0992
• DOI: 10.1016/j.jtherbio.2018.03.008

Temperature has a strong effect on ectotherm development rate. It is therefore possible to construct predictive models of development that rely solely on temperature, which have applications in a range of biological fields. Here, we leverage a reference series of development stages for embryos of the turtle Chelydra serpentina, which was described at a constant temperature of 20 °C. The reference series acts to map each distinct developmental stage onto embryonic age (in days) at 20 °C. By extension, an embryo taken from any given incubation environment, once staged, can be assigned an equivalent age at 20 °C. We call this concept “Equivalent Development”, as it maps the development stage of an embryo incubated at a given temperature to its equivalent age at a reference temperature. In the laboratory, we used the concept of Equivalent Development to estimate development rate of embryos of C. serpentina across a series of constant temperatures. Using these estimates of development rate, we created a thermal performance curve measured in units of Equivalent Development (TPCED). We then used the TPCED to predict developmental stage of embryos in several natural turtle nests across six years. We found that 85% of the variation of development stage in natural nests could be explained. Further, we compared the predictive accuracy of the model based on the TPCED to the predictive accuracy of a degree-day model, where development is assumed to be linearly related to temperature and the amount of accumulated heat is summed over time. Information theory suggested that the model based on the TPCED better describes variation in developmental stage in wild nests than the degree-day model. We suggest the concept of Equivalent Development has several strengths and can be broadly applied. In particular, studies on temperature-dependent sex determination may be facilitated by the concept of Equivalent Development, as development age maps directly onto the developmental series of the organism, allowing critical periods of sex determination to be delineated without invasive sampling, even under fluctuating temperature.
•A TPC was estimated in the laboratory for snapping turtle embryonic development.•The TPC incorporates anatomical differentiation to estimate development.•The TPC accurately predicted development in wild nests over 6 years.•It is broadly applicable and we suggest how it may be used in the future.