Effect of histological processing and methacrylate sectioning on the area of gill tissue in teleost

Deformation of biological tissues may occur during histological processing and results in loss of accuracy when quantitative information about cells, tissues and organs is necessary. In this study, the gill tissue from armored catfish (Pterygoplichthys anisitsi) was quantified in each step of proces...

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Bibliographic Details
Published in:	Brazilian journal of biology Vol. 69; no. 2; pp. 385 - 387
Main Authors:	Cruz, A L, Fernandes, M N, Perry, S F
Format:	Journal Article
Language:	English
Published:	Brazil Instituto Internacional de Ecologia 01-05-2009
Subjects:	Animals armored catfish BIOLOGY Catfishes - anatomy & histology gills Gills - anatomy & histology methacrylate Methacrylates - chemistry morphometry shrinkage Tissue Embedding - methods cascudo morfometria brânquias armored catfish morphometry methacrylate metacrilato gills shrinkage redução de área branquial
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Summary:	Deformation of biological tissues may occur during histological processing and results in loss of accuracy when quantitative information about cells, tissues and organs is necessary. In this study, the gill tissue from armored catfish (Pterygoplichthys anisitsi) was quantified in each step of processing using the stereological principles. During processing for glycol methacrylate embedding, gill tissue from shrinks significantly but regains its original dimensions after sectioning.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1519-6984 1678-4375 1678-4375 1519-6984
DOI:	10.1590/S1519-69842009000200022