Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma: upregulation of claudin-1, claudin-3, claudin-4, and β-catenin

• Published in: International journal of colorectal disease Vol. 24; no. 4; pp. 361 - 368
• Main Authors: Mees, S. T, Mennigen, R, Spieker, T, Rijcken, E, Senninger, N, Haier, J, Bruewer, M
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-04-2009; Springer-Verlag; Springer
• Subjects: Adherens Junctions - metabolism; Adult; Aged; beta Catenin - metabolism; Biological and medical sciences; Carcinoma in Situ - metabolism; Cell-cell adhesion; Claudin; Claudin-1; Claudin-3; Claudin-4; Colitis, Ulcerative - complications; Colitis, Ulcerative - metabolism; Colitis, Ulcerative - pathology; colorectal neoplasms; Colorectal Neoplasms - complications; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Demography; Female; Fluorescent Antibody Technique; Gastroenterology; Gastroenterology. Liver. Pancreas. Abdomen; Hepatology; Humans; Internal Medicine; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Male; Medical sciences; Medicine; Medicine & Public Health; Membrane Proteins - metabolism; Middle Aged; Original Article; Other diseases. Semiology; Proctology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Surgery; Tight junction; Tight Junctions - metabolism; Tumors; Ulcerative colitis; Up-Regulation; Tight junction; Claudin; Cell–cell adhesion; Colorectal cancer; Ulcerative colitis; Rectal disease; Colorectal carcinoma; Cell-cell adhesion; Malignant tumor; Protein; Inflammatory disease; Colonic disease; β Catenin; Gastroenterology; Digestive diseases; Intestinal disease; Cancer
• ISSN: 0179-1958; 1432-1262
• DOI: 10.1007/s00384-009-0653-y

Background Tight junction (TJ) proteins play a critical role in cellular adhesion, glandular differentiation, and cellular proliferation. The function of these proteins is compromised in a number of intestinal diseases, including ulcerative colitis that has an increased incidence for colorectal carcinoma (CAC). The aim of this study was to determine the expression of TJ proteins, claudin-1-4, occludin, ZO-1, and the adherens junction (AJ) protein β-catenin in CAC. Methods Sixteen colectomy specimens with CAC, adjoining intraepithelial neoplasia, and normal mucosa were studied by immunofluorescence. A semiquantitative evaluation of all investigated proteins was performed by scoring the staining intensity, and the TJ and AJ protein expression in neoplastic cells was compared to normal and intraepithelial neoplastic colonic mucosa. Results Using an intensity scoring system, mucosa of crypts and surfaces of CAC exhibited significantly elevated expression levels of claudin-1, claudin-3, claudin-4, and β-catenin compared to intraepithelial neoplasia and normal mucosa (p < 0.05). These data were confirmed by a comparative score. The expression of claudin-2, occludin, and ZO-1 showed no differences between the groups. Conclusion TJ proteins claudin-1, claudin-3, claudin-4, and the AJ protein β-catenin are overexpressed in CAC. This suggests that these proteins may become potential markers and targets in CAC.