Episomal plasmid-based generation of an iPSC line from an 83-year-old individual carrying the APOE4/4 genotype: i10984

• Published in: Stem cell research Vol. 17; no. 3; pp. 523 - 525
• Main Authors: Zulfiqar, Shadaan, Fritz, Barbara, Nieweg, Katja
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01-11-2016
• Subjects: Aged, 80 and over; Apolipoprotein E4 - genetics; B-Lymphocytes - cytology; Base Sequence; Cell Differentiation; Cell Line; Cellular Reprogramming; Dementia - genetics; Dementia - pathology; Embryoid Bodies - cytology; Embryoid Bodies - metabolism; Female; Genotype; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Karyotype; Microscopy, Fluorescence; Plasmids - genetics; Plasmids - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 1873-5061; 1876-7753
• DOI: 10.1016/j.scr.2016.09.017

In this study, lymphoblastoid cells derived from a 83-year old individual with a 15year history of progressive presenile dementia, were used to generate iPS cells, employing episomal plasmids expressing OCT4, SOX2, LIN28, L-MYC and a p53 shRNA. The individual was homozygous for the APOE4 allele. The resulting iPS cells had a normal karyotype, retained the APOE4/4 genotype, expressed pluripotency markers, were free of genomically integrated plasmids, and could be differentiated into cell type representatives from the three germ layers in vitro.