Outbreak of brainstem encephalitis associated with enterovirus-A71 in Catalonia, Spain (2016): a clinical observational study in a children's reference centre in Catalonia

• Published in: Clinical microbiology and infection Vol. 23; no. 11; pp. 874 - 881
• Main Authors: Casas-Alba, D., de Sevilla, M.F., Valero-Rello, A., Fortuny, C., García-García, J.-J., Ortez, C., Muchart, J., Armangué, T., Jordan, I., Luaces, C., Barrabeig, I., González-Sanz, R., Cabrerizo, M., Muñoz-Almagro, C., Launes, C.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-11-2017
• Subjects: Anti-Inflammatory Agents - therapeutic use; Brain Stem - virology; Brainstem; Child, Preschool; Disease Outbreaks - statistics & numerical data; Encephalitis; Encephalitis, Viral - epidemiology; Encephalitis, Viral - physiopathology; Encephalitis, Viral - therapy; Encephalitis, Viral - virology; Enterovirus; Enterovirus A, Human - genetics; Enterovirus Infections - epidemiology; Enterovirus Infections - physiopathology; Enterovirus Infections - therapy; Enterovirus Infections - virology; EV-A71; Female; Humans; Infant; Male; Molecular Epidemiology; Spain - epidemiology; Types; EV-A71; Molecular epidemiology; Encephalitis; Types; Enterovirus; Brainstem
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1016/j.cmi.2017.03.016

To describe the characteristics of an outbreak of brainstem encephalitis and encephalomyelitis related to enterovirus (EV) infection in Catalonia (Spain), a setting in which these manifestations were uncommon.
Clinical and microbiological data were analysed from patients with neurological symptoms associated with EV detection admitted to a reference paediatric hospital between April and June 2016.
Fifty-seven patients were included. Median age was 27.7 months (p25-p75 17.1–37.6). Forty-one (72%) were diagnosed with brainstem encephalitis, seven (12%) with aseptic meningitis, six (11%) with encephalitis, and three (5%) with encephalomyelitis (two out of three with cardiopulmonary failure). Fever, lethargy, and myoclonic jerks were the most common symptoms. Age younger than 12 months, higher white-blood-cell count, and higher procalcitonin levels were associated with cardiopulmonary failure. Using a PAN-EV real-time PCR, EV was detected in faeces and/or nasopharyngeal aspirate in all the patients, but it was found in cerebrospinal fluid only in patients with aseptic meningitis. EV was genotyped in 47 out of 57 and EV-A71 was identified in 40 out of 47, being the only EV type found in patients with brainstem symptoms. Most of the detected EV-A71 strains were subgenogroup C1. Intravenous immunoglobulins were used in 34 patients. Eight cases (14%) were admitted to the intensive care unit. All the patients but three, those with encephalomyelitis, showed a good clinical course and had no significant sequelae. No deaths occurred.
The 2016 outbreak of brainstem encephalitis in Catalonia was associated with EV-A71 subgenogroup C1. Despite the clinical manifestations of serious disease, a favourable outcome was observed in the majority of patients.