Exogenous allogenic fragmented double-stranded DNA is internalized into human dendritic cells and enhances their allostimulatory activity

• Published in: Cellular immunology Vol. 262; no. 2; pp. 120 - 126
• Main Authors: Alyamkina, Ekaterina A., Dolgova, Evgenia V., Likhacheva, Anastasia S., Rogachev, Vladimir A., Sebeleva, Tamara E., Nikolin, Valeriy P., Popova, Nelly A., Kiseleva, Elena V., Orishchenko, Konstantin E., Sakhno, Ludmila V., Gel’fgat, Evgeniy L., Ostanin, Alexandr A., Chernykh, Elena R., Zagrebelniy, Stanislav N., Bogachev, Sergey S., Shurdov, Mikhail A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 2010
• Subjects: Allostimulatory activity; Animals; Cytoplasm; Dendritic Cells - cytology; Dendritic Cells - immunology; DNA - immunology; DNA - ultrastructure; Endocytosis - immunology; Escherichia coli; HeLa Cells; Humans; Lung tuberculosis; Mice; Mononuclear cells; Nucleosome-free fragmented dsDNA; Lung tuberculosis; Nucleosome-free fragmented dsDNA; Allostimulatory activity; Mononuclear cells
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/j.cellimm.2010.01.005

Exogenous allogenic DNA as nucleosome-free fragments reaches main cellular compartments (cytoplasm, nucleus) of human dendritic cells and deposits in the nuclear interchromosomal space without visibly changing in linear size. The presence of such allogenic fragmented DNA in medium in which human dendritic cells are cultured produces an enhancement of their allostimulatory activity. This enhancement is comparable to that produced by the standard maturation stimulus lipopolysaccharide
Escherichia coli.