Seed priming with BABA (β-amino butyric acid): a cost-effective method of abiotic stress tolerance in Vigna radiata (L.) Wilczek

• Published in: Protoplasma Vol. 253; no. 2; pp. 277 - 289
• Main Authors: Jisha, K. C, Jos T. Puthur
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-03-2016; Springer Nature B.V
• Subjects: Aminobutyrates - pharmacology; Biomedical and Life Sciences; butyric acid; Cell Biology; chlorophyll; Chlorophyll - metabolism; cost effectiveness; drought; drought tolerance; enzyme activity; Germination; growth retardation; Life Sciences; malondialdehyde; Malondialdehyde - metabolism; Mitochondria - metabolism; mung beans; nitrate reductase; Original Article; peroxidase; Photosynthesis; Plant Sciences; polyethylene glycol; proline; protein content; salt stress; Salt-Tolerance - drug effects; seed priming; seedling growth; seedlings; Seedlings - drug effects; Seedlings - growth & development; seeds; Seeds - drug effects; Seeds - physiology; sodium chloride; stress tolerance; Stress, Physiological; superoxide dismutase; thin layer chromatography; Vigna - drug effects; Vigna - growth & development; Vigna radiata; Zoology; Mitochondrial activity; Photochemical activity; Drought; Abiotic stress; Seed priming
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-015-0804-7

The effects of β-amino butyric acid (BABA) on abiotic stress tolerance potential of three Vigna radiata varieties were studied. The reduction in the growth of seedlings subjected to NaCl/polyethylene glycol (PEG) stress is alleviated by BABA seed priming, which also enhanced photosynthetic pigment content and photosynthetic and mitochondrial activities, and also modified the chlorophyll a fluorescence-related parameters. Moreover, BABA seed priming reduced malondialdehyde content in the seedlings and enhanced the accumulation of proline, total protein, total carbohydrate, nitrate reductase activity, and activities of antioxidant enzymes like guaiacol peroxidase and superoxide dismutase. Most of these positive features of BABA priming were predominantly exhibited when the plants were encountered with stress (NaCl/PEG). The BABA content in the BABA-treated green gram seeds and seedlings was also detected and quantified with high-performance thin layer chromatography (HPTLC), and it revealed that the priming effect of BABA initiated in seeds and further gets carried over to the seedlings. It was concluded that BABA seed priming improved the drought and salinity stress tolerance potential of all the three green gram varieties, and it was evident in the NaCl-tolerant variety Pusa Vishal as compared to Pusa Ratna (abiotic stress sensitive) and Pusa 9531(drought tolerant). Dual mode in cost effectiveness of BABA priming is evident from: (1) the positive features of priming are being exhibited more during the exposure of plants to stress, and (2) priming of seedlings can be carried out by BABA application to seeds at very low concentration and volume.