Phylogenetic analysis and morphology of Ganoderma multipileum, a Ganoderma species associated with dieback of the metropolitan woody plant Delonix regia (Boj. ex Hook.) Raf. in Vietnam

• Published in: Science progress (1916) Vol. 106; no. 3; p. 368504231195503
• Main Authors: Nguyen, Trang Thi Thu, Nguyen, Hoang Duc, Bui, Anh Tu, Pham, Khanh Huyen Thi, Van, Kim Thuong Pham, Tran, Linh Thuoc, Tran, Manh Hung
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01-07-2023; Sage Publications Ltd
• Subjects: Basidiomes; Basidiospores; Biology & Life Sciences; Conditional probability; Delonix regia; Dieback; Electron microscopes; Electron microscopy; Ganoderma; Hyphae; Morphology; Mushrooms; Phenotypes; Phylogenetics; Phylogeny; Ribosomal DNA; Scanning electron microscopy; Spores; Woody plants; Vietnam; Ganodermaceae; Delonix regia dieback; wood decay mushroom; Ganoderma multipileum
• ISSN: 0036-8504; 2047-7163
• DOI: 10.1177/00368504231195503

Ganoderma multipileum, a wood decay mushroom, was initially discovered and classified in Taiwan through the analysis of its morphology and the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. In this study, we identified a mushroom associated with the dieback of Delonix regia (Boj. ex Hook.) Raf., a woody ornamental street tree in Vietnam, as Ganoderma multipileum. This classification was based on phylogenetic analysis of ITS, RPB2, and TEF1 sequences, as well as morphology assessment and scanning electron microscope observation of basidiospores. The phylogenetic analysis revealed that the specimens collected in Vietnam formed a monophyletic group of Ganoderma multipileum with a high bootstrap value and posterior probability (100%/1.00). Furthermore, the morphological features consistent with laccate Ganoderma, including a thin pileipellis composed of enlarged and bulbous hyphae, and the basidiomes exhibited two different phenotypes. Notably, scanning electron microscopy of the basidiospores revealed ovoid spores with numerous echinules, providing the first documented evidence of this characteristic for Ganoderma multipileum. This research represents the first recorded instance of Ganoderma multipileum in Vietnam associated with the dieback of Delonix regia.