Doxorubicin-loaded pH-responsive chitin nanogels for drug delivery to cancer cells

• Published in: Carbohydrate polymers Vol. 87; no. 3; pp. 2352 - 2356
• Main Authors: Jayakumar, R., Nair, Amrita, Rejinold, N. Sanoj, Maya, S., Nair, S.V.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 14-02-2012; Elsevier
• Subjects: Antineoplastic agents; Applied sciences; Biological and medical sciences; Cancer drug delivery; Carbohydrates; Cellular uptake; Chemotherapy; Chitin; Cytotoxicity; Doxorubicin; Doxorubicin loaded chitin nanogels; Drug delivery; Exact sciences and technology; Fourier transform infrared spectroscopy; Liver cancer; liver neoplasms; Lung cancer; Medical sciences; Natural polymers; neoplasm cells; pH responsivity; Pharmacology. Drug treatments; Physicochemistry of polymers; Prostate; scanning electron microscopy; Starch and polysaccharides; Swelling; Toxicity; Cancer drug delivery; Cellular uptake; Doxorubicin loaded chitin nanogels; Swelling; pH responsivity; Antineoplastic agent; Control release polymer; Drug carrier; Chitin; Experimental study; Doxorubicin; In vitro; Nanoencapsulation; Biological activity; Particle size distribution; Nanogel; Internalization; Oside polymer; Manufacturing; Tumor cell
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2011.10.040

► We developed doxorubicin loaded pH responsive chitin nanogels (Dox-CNGs) for cancer drug delivery applications. ► The in vitro cytotoxicity studies of 130–160nm sized Dox-CNGs proved their cyto compatibility with L929 and toxicity towards PC3, MCF-7, A549 and HEPG2 cancer cells. ► The internalization of the Dox-CNGs showed a significant uptake of the Dox-CNGs in all the tested cell lines. ► These results indicated that Dox-CNGs can be used for prostate, breast, lung and liver cancer.
This work deals with preparation of doxorubicin loaded chitin nanogels and were characterized by SEM, DLS and FTIR for cancer drug delivery. The in vitro cytotoxicity studies of 130–160nm sized doxorubicin loaded chitin nanogels were studied using MTT assay on L929, PC3, MCF-7, A549 and HEPG2 confirmed that relatively higher toxicity on cancer cells comparing to normal L929 cells. The internalization studies showed a significant uptake of doxorubicin loaded chitin nanogels in all the tested cell lines. All the above results indicated that doxorubicin loaded chitin nanogels can be used for prostate, breast, lung and liver cancer.