Neuron-specific enolase-cre mouse line with cre activity in specific neuronal populations

• Published in: Genesis (New York, N.Y. : 2000) Vol. 44; no. 3; pp. 130 - 135
• Main Authors: Kwon, Chang-Hyuk, Zhou, Jing, Li, Yanjiao, Kim, Ki Woo, Hensley, Lori L., Baker, Suzanne J., Parada, Luis F.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-03-2006
• Subjects: Animals; brain; Cerebral Cortex - enzymology; cre; Female; Gene Expression Regulation, Enzymologic; Hippocampus - enzymology; Integrases - metabolism; Kidney - enzymology; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; mouse; nervous system; neuron; neuron-specific enolase; Neurons - enzymology; Phosphopyruvate Hydratase - metabolism; Testis - enzymology
• ISSN: 1526-954X; 1526-968X
• DOI: 10.1002/gene.20197

To establish genetic tools for conditional gene deletion in mouse neurons, we generated two independent neuron‐specific enolase (Nse)‐cre transgenic lines. The transgenic line termed Nse‐creCK1 showed cre activity in most neuronal regions in the nervous system, while the Nse‐creCK2 line exhibited a unique cre activity that has not been reported in other cre transgenic lines. Nse‐creCK2 cre activity was detectable from embryogenesis and mostly restricted to neuronal regions. In postnatal brain, the Nse‐creCK2 line exhibited cre activity limited to differentiated neurons in the cerebral cortex and hippocampus. Cre activity was assayed in several internal organs and sporadic activity was limited to the kidney and testis. We conclude that these cre lines will be useful for studying loss of gene function in specific neuronal populations. genesis 44:130–135, 2006. Published 2006 Wiley‐Liss, Inc.