Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China

• Published in: Virus research Vol. 106; no. 1; pp. 15 - 26
• Main Authors: Yang, H., Makeyev, E.V., Kang, Z., Ji, S., Bamford, D.H., van Dijk, A.A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-11-2004
• Subjects: ADRV-N; Adult; Capsid Proteins - genetics; China; Cloning, Molecular; Disease Outbreaks; Double-stranded RNA binding motif (DSRM); Gastroenteritis - epidemiology; Gastroenteritis - virology; Genome segment 5, 6, 7 nucleotide sequences; Genome, Viral; Humans; Non-group A, B, C rotavirus; Novel adult diarrhoea rotavirus; NSP1; NSP3; Phylogeny; RNA, Double-Stranded - analysis; RNA, Viral - analysis; RNA, Viral - genetics; Rotavirus; Rotavirus - chemistry; Rotavirus - classification; Rotavirus - genetics; Rotavirus Infections - epidemiology; Rotavirus Infections - virology; Sequence Analysis; VP6; China; NSP1; ADRV-N; Double-stranded RNA binding motif (DSRM); Genome segment 5, 6, 7 nucleotide sequences; Non-group A, B, C rotavirus; Novel adult diarrhoea rotavirus; NSP3; VP6
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/j.virusres.2004.05.011

A diarrhoeal outbreak among adults in China was caused by a new rotavirus, termed ADRV-N, that does not react with antisera directed against group A, B or C rotaviruses [Zhonghua Liu Xing Bing Xue Za Zhi (Chin. Epidemiol.) 19 (1998) 336]. ADRV-N can be propagated in cell cultures [Zhonghua Yi Xue Za Zhi (Natl. Med. J. China) 82 (2002) 14]. We present the complete sequences for ADRV-N genome segments 5 and 6, and a full ORF sequence of genome segment 7. The deduced amino acid sequences suggest that these segments encode NSP1, VP6 and NSP3, respectively. These three ADRV-N genome segments have a unique -ACCCC-3′ terminal sequence. The 5′-GG- terminus of segments 5 and 6 is the same as that of other rotaviruses. The amino acid similarity between VP6 and NSP3 of ADRV-N and the cognate sequences of their closest counterpart, group B IDIR, was 37 and 35%, respectively. The ADRV-N NSP1 has a double-stranded RNA binding motif (DSRM) and a putative autoproteolytic cleavage motif upstream from the DSRM. The putative ADRV-N NSP3 has a truncated C-terminus compared to the cognate protein of group B rotaviruses. All the available data demonstrate that ADRV-N differs significantly from the known rotaviruses and strongly suggest that ADRV-N is the first recognized member of a new group of rotaviruses infecting humans.