Acoustic Characterization of the CLINIcell for Ultrasound Contrast Agent Studies

• Published in: IEEE transactions on ultrasonics, ferroelectrics, and frequency control Vol. 66; no. 1; pp. 244 - 246
• Main Authors: Beekers, Ines, van Rooij, Tom, van der Steen, Antonius F. W., de Jong, Nico, Verweij, Martin D., Kooiman, Klazina
• Format: Journal Article
• Language: English
• Published: United States IEEE 01-01-2019; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Acoustic characterization; Acoustics; Amplitudes; Biomedical optical imaging; Biotechnology; Cell culture; Contrast agents; Drug delivery; Drug delivery systems; Integrated optics; Optical sensors; Optical variables control; ultrahigh-speed imaging; Ultrasonic imaging; ultrasound contrast agents (UCAs)
• ISSN: 0885-3010; 1525-8955
• DOI: 10.1109/TUFFC.2018.2881724

Ultrasound contrast agents consist of gas-filled coated microbubbles that oscillate upon ultrasound insonification. Their characteristic oscillatory response provides contrast enhancement for imaging and has the potential to locally enhance drug delivery. Since microbubble response depends on the local acoustic pressure, an ultrasound compatible chamber is needed to study their behavior and the underlying drug delivery pathways. In this study, we determined the amplitude of the acoustic pressure in the CLINIcell, an optically transparent chamber suitable for cell culture. The pressure field was characterized based on microbubble response recorded using the Brandaris 128 ultrahigh-speed camera and an iterative processing method. The results were compared to a control experiment performed in an OptiCell, which is conventionally used in microbubble studies. Microbubbles in the CLINIcell responded in a controlled manner, comparable to those in the OptiCell. For frequencies from 1 to 4 MHz, the mean pressure amplitude was −5.4 dB with respect to the externally applied field. The predictable ultrasound pressure demonstrates the potential of the CLINIcell as an optical, ultrasound, and cell culture compatible device to study microbubble oscillation behavior and ultrasound-mediated drug delivery.