A sequence-tagged site map of human chromosome 11

• Published in: Genomics (San Diego, Calif.) Vol. 17; no. 3; p. 699
• Main Authors: Smith, M W, Clark, S P, Hutchinson, J S, Wei, Y H, Churukian, A C, Daniels, L B, Diggle, K L, Gen, M W, Romo, A J, Lin, Y
• Format: Journal Article
• Language: English
• Published: United States 01-09-1993
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping; Chromosomes, Human, Pair 11; Cloning, Molecular; Cosmids; Cricetinae; DNA Primers - genetics; Exons; Genetic Markers; Humans; Hybrid Cells; Mice; Molecular Sequence Data; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Sequence Homology, Amino Acid; Sequence Tagged Sites
• ISSN: 0888-7543
• DOI: 10.1006/geno.1993.1392

We report the construction of 370 sequence-tagged sites (STSs) that are detectable by PCR amplification under sets of standardized conditions and that have been regionally mapped to human chromosome 11. DNA sequences were determined by sequencing directly from cosmid templates using primers complementary to T3 and T7 promoters present in the cloning vector. Oligonucleotide PCR primers were predicted by computer and tested using a battery of genomic DNAs. Cosmids were regionally localized on chromosome 11 by using fluorescence in situ hybridization or by analyzing a somatic cell hybrid panel. Additional STSs corresponding to known genes and markers on chromosome 11 were also produced under the same series of standardized conditions. The resulting STSs provide uniform coverage of chromosome 11 with an average spacing of 340 kb. The DNA sequence determined for use in STS production corresponds to about 0.1% (116 kb) of chromosome 11 and has been analyzed for the presence of repetitive sequences, similarities to known genes and motifs, and possible exons. Computer analysis of this sequence has identified and therefore mapped at least eight new genes on chromosome 11.