Ultrastructural and biochemical investigations of protein mobilization of Mucuna pruriens (L.) DC. cotyledons and embryo axis

• Published in: Protoplasma Vol. 239; no. 1-4; pp. 15 - 21
• Main Authors: Muccifora, Simonetta, Guerranti, Roberto, Muzzi, Chiara, Hope-Onyekwere, Nnadozies S, Pagani, Roberto, Leoncini, Roberto, Bellani, Lorenza M
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01-03-2010; Springer Vienna; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Cell Biology; Cotyledon - chemistry; Cotyledon - metabolism; Cotyledon - ultrastructure; Humans; Life Sciences; Mucuna - anatomy & histology; Mucuna - chemistry; Mucuna - embryology; Original Article; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Proteins; Seeds - metabolism; Seeds - ultrastructure; Zoology; SDS-PAGE; Ultrastructure; Germination
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-009-0076-1

The mobilization of storage reserves, with particular emphasis on storage proteins of Mucuna pruriens (L.) DC., cotyledons, and embryo was investigated from the ultrastructural and biochemical points of view. Proteins and starch were the two main storage substances in cotyledons, and proteins and lipids were the main ones in the embryo. Embryo protein bodies were smaller and fewer in number than those of cotyledons. Structural and ultrastructural data determined between 24 and 48 h after imbibition and between 48 and 72 h after imbibition, the end of significant embryo and cotyledon protein mobilization, respectively, indicating more precocious storage protein mobilization in the axis than cotyledons. Moreover, storage protein mobilization in embryo and cotyledons occurred before the end of germination. Water soluble proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, producing 29 bands with molecular weights from 14 to 90 KDa. Embryo extract contained more proteins than cotyledon extract, contained seven characteristic bands, and showed a higher variability of the optical density trend than cotyledon.