Tumebacillus algifaecis sp. nov., isolated from decomposing algal scum

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. 7; pp. 2194 - 2198
• Main Authors: Wu, Yu-Fan, Zhang, Bo, Xing, Peng, Wu, Qing-Long, Liu, Shuang-Jiang
• Format: Journal Article
• Language: English
• Published: England 01-07-2015
• Subjects: Alicyclobacillus - classification; Alicyclobacillus - genetics; Alicyclobacillus - isolation & purification; Bacterial Typing Techniques; Base Composition; China; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; Eutrophication; Fatty Acids - chemistry; Lakes; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan - chemistry; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; China
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.000240

Bacterial strain THMBR28(T) was isolated from decomposing algal scum that was collected during an algal bloom in Taihu lake, China. Cells of strain THMBR28(T) were Gram-staining-positive, facultatively anaerobic and rod-shaped. Growth was observed at 20-45 °C (optimum, 30 °C), at pH 5.0-9.5 (optimum, pH 6.5-7.5), and in the presence of 0-1.0% (w/v) NaCl (optimum, 0.5%). Strain THMBR28(T) contained MK-7 as the major menaquinone and iso-C15 : 0 as the major cellular fatty acid. The polar lipid profile contained phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and six unidentified polar lipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The DNA G+C content was 57.6 mol% (Tm). Phylogenetic analysis of 16S rRNA gene sequences showed that strain THMBR28(T) belonged to the genus Tumebacillus, most closely related to Tumebacillus ginsengisoli DSM 18389(T) (95.0%) and Tumebacillus permanentifrigoris Eur1 9.5(T) (93.4%). Based on phylogenetic and phenotypic characterization, it is concluded that strain THMBR28(T) represents a novel species of the genus Tumebacillus, for which the name Tumebacillus algifaecis sp. nov. is proposed, with THMBR28(T) ( = CGMCC 1.10949(T) = NBRC 108765(T)) as the type strain.