Triggering the Electrolyte-Gated Organic Field-Effect Transistor output characteristics through gate functionalization using diazonium chemistry: Application to biodetection of 2,4-dichlorophenoxyacetic acid

• Published in: Biosensors & bioelectronics Vol. 113; pp. 32 - 38
• Main Authors: Nguyen, T.T.K., Nguyen, T.N., Anquetin, G., Reisberg, S., Noël, V., Mattana, G., Touzeau, J., Barbault, F., Pham, M.C., Piro, B.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15-08-2018
• Subjects: 2,4-D; 2,4-dichlorophenoxyacetic acid; Diazonium salt; Electrolyte-Gated OFET; Immunosensor; Molecular modelling; 2,4-dichlorophenoxyacetic acid; Electrolyte-Gated OFET; Diazonium salt; Molecular modelling; 2,4-D; Immunosensor
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2018.04.051

We investigated an Electrolyte-Gated Organic Field-Effect transistor based on poly(N-alkyldiketopyrrolo-pyrrole dithienylthieno[3,2-b]thiophene) as organic semiconductor whose gate electrode was functionalized by electrografting a functional diazonium salt capable to bind an antibody specific to 2,4-dichlorophenoxyacetic acid (2,4-D), an herbicide well-known to be a soil and water pollutant. Molecular docking computations were performed to design the functional diazonium salt to rationalize the antibody capture on the gate surface. Sensing of 2,4-D was performed through a displacement immunoassay. The limit of detection was estimated at around 2.5 fM.
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•Gate functionalization of an Electrolyte-Gated Organic Field-Effect Transistor by diazonium electrografting.•Immobilization of antibodies on the transistor's gate.•Displacement immunoassay for 2,4-D detection in water samples.