Characterization of substance P-membrane interaction by transferred nuclear Overhauser effect

• Published in: Biopolymers Vol. 54; no. 5; pp. 297 - 306
• Main Authors: Augé, S., Bersch, B., Tropis, M., Milon, A.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 15-10-2000
• Subjects: Base Sequence; Deuterium; distance geometry; lipid bilayer; Liposomes - chemistry; Liposomes - metabolism; Models, Molecular; Nonlinear Dynamics; Nuclear Magnetic Resonance, Biomolecular - methods; Protein Structure, Tertiary; Substance P - chemistry; Substance P - metabolism; tachykinins; transferred nuclear Overhauser enhancement
• ISSN: 0006-3525; 1097-0282
• DOI: 10.1002/1097-0282(20001015)54:5<297::AID-BIP10>3.0.CO;2-9

Substance P, one of the mammalian tachykinins, is known to interact strongly with lipid bilayers and this interaction may play a role in the receptor–peptide recognition process. The conformation of substance P bound to vesicles consisting of perdeuterated phosphatidylcholine has been investigated by means of two‐dimensional transferred nuclear Overhauser (trNOE) spectroscopy. Nuclear magnetic resonance data analysis resulted in a unique conformational family characterized by a well‐defined conformation of the last seven C‐terminal amino acids, which consists of a sequence of nonstandard turns following each other in a helix‐like manner. The absence of short‐ or medium‐range trNOE in the N‐terminal part indicates its structural flexibility. © 2000 John Wiley & Sons, Inc. Biopoly 54: 297–306, 2000