Serum Soluble Mediator Profiles and Networks During Acute Infection With Distinct DENV Serotypes

• Published in: Frontiers in immunology Vol. 13; p. 892990
• Main Authors: Coutinho-da-Silva, Mikelly Santos, Sucupira, Pedro Henrique Ferreira, Bicalho, Kelly Alves, Campi-Azevedo, Ana Carolina, Brito-de-Sousa, Joaquim Pedro, Peruhype-Magalhães, Vanessa, Rios, Maria, Teixeira-Carvalho, Andréa, Coelho-dos-Reis, Jordana Grazziela Alves, Antonelli, Lis Ribeiro do Valle, Rezende, Vitor Bortolo de, Melo, Fernanda Ludolf Ribeiro de, Garcia, Cristiana Couto, Silva-Andrade, Jesuanne Carla, Costa-Rocha, Ismael Artur da, Bastos, Michele de Souza, Rocha, Lucia Alves da, Silva, Valderjane Aprigio, Ferreira, Ewerton da Silva, Marinho, Eveny Perlize Melo, Costa, Allyson Guimarães, Gomes, Matheus de Souza, Amaral, Laurence Rodrigues, Furtado, Erilene Cristina da Silva, Silva, Eliana Vieira Pinto da, Ramos, Bruna Alves, Santos, Éder Barros dos, Freitas, Maria Nazaré Oliveira, Vasconcelos, Pedro Fernando da Costa, Martins-Filho, Olindo Assis, Araújo, Márcio Sobreira Silva, Ferreira, Milene Silveira, Martins, Livia Carício
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 31-05-2022
• Subjects: chemokines; cytokines; dengue infection; dengue virus; DENV serotypes; immune response; Immunology
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2022.892990

A panoramic analysis of chemokines, pro-inflammatory/regulatory cytokines, and growth factors was performed in serum samples from patients with acute DENV infection (n=317) by a high-throughput microbeads array. Most soluble mediators analyzed were increased in DENV patients regardless of the DENV serotype. The substantial increase (≥10-fold) of CXCL10, IL-6, and IFN-γ, and decreased levels of PDGF (<0.4-fold) was universally identified in all DENV serotypes. Of note, increased levels of CXCL8, CCL4, and IL-12 (≥3-9-fold) were selectively observed in DENV2 as compared to DENV1 and DENV4. Heatmap and biomarker signatures further illustrated the massive release of soluble mediators observed in DENV patients, confirming the marked increase of several soluble mediators in DENV2. Integrative correlation matrices and networks showed that DENV infection exhibited higher connectivity among soluble mediators. Of note, DENV2 displayed a more complex network, with higher connectivity involving a higher number of soluble mediators. The timeline kinetics (Day 0-1, D2, D3, D4-6) analysis additionally demonstrated differences among DENV serotypes. While DENV1 triggers a progressive increase of soluble mediators towards D3 and with a decline at D4-6, DENV2 and DENV4 develop with a progressive increase towards D4-6 with an early plateau observed in DENV4. Overall, our results provided a comprehensive overview of the immune response elicited by DENV infection, revealing that infection with distinct DENV serotypes causes distinct profiles, rhythms, and dynamic network connectivity of soluble mediators. Altogether, these findings may provide novel insights to understand the pathogenesis of acute infection with distinct DENV serotypes.