Tissue Microbiome of Norway Spruce Affected by Heterobasidion-Induced Wood Decay

• Published in: Microbial ecology Vol. 77; no. 3; pp. 640 - 650
• Main Authors: Ren, Fei, Kovalchuk, Andriy, Mukrimin, Mukrimin, Liu, Mengxia, Zeng, Zhen, Ghimire, Rajendra P., Kivimäenpää, Minna, Holopainen, Jarmo K., Sun, Hui, Asiegbu, Fred O.
• Format: Journal Article
• Language: English
• Published: New York Springer Science + Business Media 01-04-2019; Springer US; Springer Nature B.V
• Subjects: Abiotic factors; Bacteria; Bark; Biomedical and Life Sciences; Composition effects; Ecology; Evergreen trees; Fitness; Geoecology/Natural Processes; Life Sciences; Microbial activity; Microbial Ecology; Microbiology; Microbiomes; Microorganisms; Monoterpenes; Nature Conservation; Pathogens; Picea abies; Pine needles; Pine trees; Plant communities; PLANT MICROBE INTERACTIONS; Profiles; Sesquiterpenes; Surveying; Symbionts; Terpenes; Tissue; Tissues; Trees; Water Quality/Water Pollution; Wood; Xylem; Norway; Host-microbe interactions; Norway spruce; Terpenoids; Bacterial biota; Microbial community; Microbiome; Heterobasidion
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-018-1240-y

Plants live in close association with microbial symbionts, which may affect the host fitness, productivity, and tolerance against biotic and abiotic stressors. The composition of plant microbial communities is influenced by many biotic and abiotic factors, but little is known about the effect of plant pathogens on the structure of these communities. In this study, we investigated the structure of bacterial communities associated with different tissues of asymptomatic and symptomatic (Heterobasidion-rotten) Norway spruce (Picea abies (L.) Karst.) trees. Our results demonstrated that each of the investigated anatomic tissues (root, bark, down stem, upper stem, and needles) harbored a unique bacterial assemblage. However, the health status of the host trees had little effect on the structure of bacterial communities, as the only significant differences among asymptomatic and symptomatic trees were found in the composition of the bacterial communities of needles. Proteobacteria was predominant in all anatomic regions with the highest abundance in needles (86.7 %), whereas Actinobacteria showed an opposite trend, being more abundant in the woody tissues than in needles. Additionally, we performed profiling of terpenoid compounds present in spruce xylem and phloem. Total concentrations of monoterpenes and sesquiterpenes were considerably higher in asymptomatic trees. However, we found no significant correlations between terpenoid profiles of spruce trees and the composition of their bacterial communities. Our results provide an insight into the diversity of bacteria associated with Norway spruce tree tissues. At the same time, the health status and terpenoid content of host trees had a limited effect on the composition of bacterial communities in our survey.