Social Stress during the Estrus or Luteal Phase in Sheep

• Published in: Journal of applied animal welfare science Vol. 27; no. 1; pp. 12 - 20
• Main Authors: Freitas-de-Melo, Aline, Garcia Kako Rodriguez, Mariana, Crosa, Camila, Ungerfeld, Rodolfo
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 2024; Taylor & Francis Ltd
• Subjects: Animals; Blood proteins; Cortisol; Estrogens; Estrus - physiology; Estrus cycle; Female; Globulins; Glucose; Hormones; Hydrocortisone; Luteal Phase - physiology; Mammals; open field test; Physiology; Progesterone; Proteins; Reproductive status; Serum proteins; Sheep; Social interactions; steroid; Stress response; Surface temperature; glucose; open field test; surface temperature; Blood proteins; steroid
• ISSN: 1088-8705; 1532-7604
• DOI: 10.1080/10888705.2021.2021408

In mammals, progesterone and estrogens affect the stress response. The study aimed to compare the physiological and behavioral responses to the social isolation of ewes during the estrus or luteal phase. Estrous and diestrous ewes (n = 10 and 8 respectively) were individually isolated in a novel place for 10 min. Ewes' behavior was recorded during the test. Cortisol, blood proteins and glucose concentrations, and the skin surface temperature were determined before and after the test. Cortisol increased immediately after the test ended (P = 0.02). Serum total protein (P = 0.02), globulin (P < 0.0001), and plasma glucose (P = 0.006) concentrations were greater in estrus than in the luteal phase. The abdominal skin surface temperature was greater during estrus than during the luteal phase (P = 0.02). Ewes in estrus spent more time standing up immobile than ewes in the luteal phase (P = 0.05). In conclusion, the physiological and behavioral responses changed according to the phase of the estrous cycle. These results highlight the need for future studies focusing on how reproductive status influences the stress response to different management practices in sheep.