Anti-glycation effect and the α-amylase, lipase, and α-glycosidase inhibition properties of a polyphenolic fraction derived from citrus wastes

• Published in: Preparative biochemistry & biotechnology Vol. 50; no. 8; pp. 794 - 802
• Main Authors: Fernandes, Annayara C. F., Santana, Ádina L., Martins, Isabela M., Moreira, Debora K. T., Macedo, Juliana A., Macedo, Gabriela A.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-09-2020; Taylor & Francis Ltd
• Subjects: Advanced glycosylation end products; Age; alpha-Amylases - antagonists & inhibitors; Amino groups; Amylases; Animals; Antiglycation; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; biowaste; Cattle; Chronic illnesses; Citrus - chemistry; Digestive enzymes; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; flavonoids; Glucosidase; Glycation End Products, Advanced - antagonists & inhibitors; Glycosidases; Glycoside Hydrolases - antagonists & inhibitors; Glycosylation; Glycosylation - drug effects; Hesperidin; inhibition of digestive enzymes; Lipase; Lipase - antagonists & inhibitors; Pancreas; Pathogenesis; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Polyphenols - chemistry; Polyphenols - isolation & purification; Polyphenols - pharmacology; Saccharomyces cerevisiae - enzymology; Sugar; Swine; Wastes; α-Amylase; α-Glucosidase; antioxidant; Antiglycation; biowaste; inhibition of digestive enzymes; flavonoids
• ISSN: 1082-6068; 1532-2297
• DOI: 10.1080/10826068.2020.1737941

The advanced glycation end products (AGEs) constitute a wide variety of substances synthesized from interactions between amino groups of proteins and reducing sugars, which excess induces pathogenesis of chronic diseases. Brazil is the major producer of citrus, a low-cost source of hesperidin, which is a polyphenol recognized for its capacity to inhibit AGEs formation. This is the first work to evaluate the effects of a polyphenolic fraction derived from citrus wastes on the antiglycation and on the inhibition properties of digestive enzymes on the possibility to process these wastes in high value-added products. At concentrations of 10, 15 and 20 mg/mL inhibition of AGEs was higher than 60%. The extracts were able to inhibit by 76% the activity of pancreatic lipase and by 98% the activity of α-glucosidase. For the α-amylase the inhibition capacity was lower than 50%. Strong correlation was obtained among anti-glycation with polyphenolic content and antioxidant capacity.